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Literature summary for 3.2.1.8 extracted from

  • Huang, Y.; Zheng, X.; Pilgaard, B.; Holck, J.; Muschiol, J.; Li, S.; Lange, L.
    Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea (2019), Appl. Microbiol. Biotechnol., 103, 777-791 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Pichia pastoris Rhizophlyctis rosea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 beechwood xylan Km: 18.83 mg/ml, pH 7, 50°C, large enzyme form RrXyn11A L Rhizophlyctis rosea
additional information
-
 beechwood xylan Km: 21.63 mg/ml, pH 7, 40°C, small enzyme form RrXyn11A S Rhizophlyctis rosea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 calculated from sequence Rhizophlyctis rosea

Organism

Organism UniProt Comment Textmining
Rhizophlyctis rosea A0A2U8LMQ3
-
-
Rhizophlyctis rosea NBRC 105426 A0A2U8LMQ3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme is not glycosylated Rhizophlyctis rosea

Purification (Commentary)

Purification (Comment) Organism
-
 Rhizophlyctis rosea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside
-
 Rhizophlyctis rosea 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside
-
 Rhizophlyctis rosea NBRC 105426 4-nitrophenol + beta-D-xylopyranose
-
 ?
azurin-cross-linked arabinoxylan + H2O
-
 Rhizophlyctis rosea ?
-
 ?
azurin-cross-linked arabinoxylan + H2O
-
 Rhizophlyctis rosea NBRC 105426 ?
-
 ?
azurin-cross-linked xylan + H2O
-
 Rhizophlyctis rosea ?
-
 ?
azurin-cross-linked xylan + H2O
-
 Rhizophlyctis rosea NBRC 105426 ?
-
 ?
beechwood xylan + H2O
-
 Rhizophlyctis rosea ?
-
 ?
beechwood xylan + H2O
-
 Rhizophlyctis rosea NBRC 105426 ?
-
 ?

Synonyms

Synonyms Comment Organism
GH11 xylanase
-
 Rhizophlyctis rosea
RrXyn11A
-
 Rhizophlyctis rosea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 small enzyme form RrXyn11A S Rhizophlyctis rosea
50
-
 large enzyme form RrXyn11A L Rhizophlyctis rosea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1 h, 56% residual activity (small enzyme form RrXyn11A S), 1 h, 65% residual activity (larger enzyme form RrXyn11A L) Rhizophlyctis rosea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54.8
-
 beechwood xylan pH 7, 50°C, large enzyme form RrXyn11A L Rhizophlyctis rosea
106.25
-
 beechwood xylan pH 7, 40°C, small enzyme form RrXyn11A S Rhizophlyctis rosea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhizophlyctis rosea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable Rhizophlyctis rosea