Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoic acid) | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requires Co2+ for activity. Cobalt is bound to the nucleosidase with a stoichiometry of 1 equivalent of cobalt/subunit | Glycine max | |
Co2+ | required for catalytic function | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59500 | - |
3 * 59500, SDS-PAGE | Glycine max |
180000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-deoxy-5'-methylthioadenosine + H2O | Glycine max | - |
? | - |
? | |
5'-methylthioadenosine + H2O | Glycine max | - |
adenine + S-methyl-5-thio-D-ribose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, acetone precipitation, Sephacryl S-200 gel filtration, DEAE-Sephacel gel filtration, and 5'-AMP-Sepharose column chromatography | Glycine max |
native enzyme 675.2fold from cotyledons by ammonium sulfate fractionation, acetone precipitation, gel filtration, anion exchange chromatography, and affinity chromatography | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.025 | - |
crude extract, at pH 7.0 and 37°C | Glycine max |
16.88 | - |
purified native enzyme, pH 7.0, 37°C | Glycine max |
16.88 | - |
after 675.2fold purification, at pH 7.0 and 37°C | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-deoxy-5'-methylthioadenosine + H2O | - |
Glycine max | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Glycine max | adenine + S-methyl-5-thio-D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 59500, SDS-PAGE | Glycine max |
trimer | 3 * 59500, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
5'-deoxy-5'-methylthioadenosine nucleosidase | - |
Glycine max |
MTA nucleosidase | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Glycine max |