Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme with or without Zn2+, hanging drop vapour diffusion method, method screening, 0.006 ml protein solution against 1 ml reservoir solution, which contains2.0-2.5 M ammonium sulfate, 14-60 days, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution, multiwavelength anomalous diffraction | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dinuclear zinc active center | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46425 | - |
1 * 46425, sequence calculation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ion exchange chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Bacillus subtilis | L-Leu + 4-nitroaniline | - |
? | |
additional information | the enzyme prefers large and hydrophobic amino acids in peptide and protein substrates | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46425, sequence calculation | Bacillus subtilis |
More | three-dimensional structure analysis | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
BSAP | - |
Bacillus subtilis |
extracellular aminopeptidase | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
20 min, purified recombinant enzyme, stable | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Bacillus subtilis |