Literature summary for 3.4.11.10 extracted from

Sarnovsky, R.; Rea, J.; Makowski, M.; Hertle, R.; Kelly, C.; Antignani, A.; Pastrana, D.V.; Fitzgerald, D.J.
Proteolytic cleavage of a C-terminal prosequence, leading to autoprocessing at the N terminus, activates leucine aminopeptidase from Pseudomonas aeruginosa (2009), J. Biol. Chem., 284, 10243-10253.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	full-length LAP is activated in a two-step process: proteolytic cleavage at the C terminus is followed by an intramolecular autocatalytic removal of a 12-amino acid propeptide at the N terminus	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Star(DE3) cells	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
D308A	inactive	Pseudomonas aeruginosa
D369A	inactive	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	0.05 mM, required for activity	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	active truncated recombinant enzyme, gel filtration	Pseudomonas aeruginosa
54930	-	recombinant enzyme, MALDI-TOF mass spectrometry	Pseudomonas aeruginosa
66000	-	inactive full-length recombinant enzyme, gel filtration	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and Superdex 200 gel filtration	Pseudomonas aeruginosa

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant LAP is refolded by adding guanidine-solubilized protein to the following solution: 0.5 M L-Arg, 50 mM Tris, pH 8.0, 1 mM CaCl2, and 0.05 mM ZnCl2	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Leu-4-nitroanilide + H2O	-	Pseudomonas aeruginosa	Leu + 4-nitroaniline	-	?
additional information	LAP shows preference for leucine and methionine over other substrates	Pseudomonas aeruginosa	?	-	?

Synonyms

Synonyms	Comment	Organism
LAP	-	Pseudomonas aeruginosa
leucine aminopeptidase	-	Pseudomonas aeruginosa
rLAP55	recombinant enzyme, full-length rLAP55 is enzymatically inactive	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)