Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-dithiothreitol | - |
Bacillus subtilis | |
EDTA | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
leucine-4-nitroaniline | pH 8.5, 60°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits activity | Bacillus subtilis | |
Ca2+ | weakly inhibits activity | Bacillus subtilis | |
Co2+ | enhances activity | Bacillus subtilis | |
Cu2+ | weakly inhibits activity | Bacillus subtilis | |
Ni2+ | inhibits activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme contains three potential glycosylation sites | Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
using ion-exchange chromatography, ultrafiltration and gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leucine-4-nitroaniline + H2O | enzyme also shows activity towards Arg-4-nitroaniline, Lys-4-nitroaniline and Met-4-nitroaniline | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant protein | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | recombinant protein | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant protein | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | recombinant protein | Bacillus subtilis |