Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
purified recombinant free AnCE and in complex with six antihypertensive drugs, captopril, enalaprilat, lisinopril, ramiprilat, trandolaprilat, perindoprilat, lisW-S, RXPA380, and RXP407, hanging drop vapour diffusion method at 16 °C, 0.002 ml of protein solution containing 10 mg/ml native AnCE in 5 mM HEPES, pH 7.5, 0.1 mM PMSF, and 0.01 mM zinc acetate, are mixed with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, and 1.5 M sodium citrate, for enzyme complexes, the crystals are mixed with inhibitor solution, X-ray diffraction structue determination and analysis at 1.85-2.10 A resolution | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
captopril | - |
Drosophila melanogaster | |
enalaprilat | - |
Drosophila melanogaster | |
lisinopril | - |
Drosophila melanogaster | |
lisW-S | lisinopril-tryptophan S-enantiomer, a C-domain human sACE specific inhibitor and antihypertensive drug | Drosophila melanogaster | |
perindoprilat | - |
Drosophila melanogaster | |
ramiprilat | - |
Drosophila melanogaster | |
RXP407 | i.e. Ac-Asp-L-Phe(PO2CH2)-L-Ala-Ala-NH2, a human sACE domain-specific phosphinic peptidyl inhibitor and antihypertensive drug | Drosophila melanogaster | |
RXPA380 | i.e. (2S)-2-[([2-[(1R)-1-[((benzyloxy)carbonyl)amino]-2-(phenylethyl)(hydroxyl)-phosphinyl]cyclopentyl]carbonyl)amino]-3-(1H-indo-3-yl)-propionic acid (Cbz-PhePSI[P(O)(OH)CH]Pro-Trp-OH), a human sACE domain-specific phosphinic peptidyl inhibitor and antihypertensive drug | Drosophila melanogaster | |
trandolaprilat | - |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | AnCE is a single domain protein with ACE activity, an ACE homologue | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q10714 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris by hydrophobic interaction chromatography and gel filtration to homogeneity | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AnCE is a single domain protein with ACE activity, an ACE homologue | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACE | - |
Drosophila melanogaster |
ANCE | - |
Drosophila melanogaster |
angiotensin I-converting enzyme | - |
Drosophila melanogaster |
angiotensin-converting enzyme | - |
Drosophila melanogaster |