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Literature summary for 3.4.16.4 extracted from

  • Rioseras, B.; Yaguee, P.; Lopez-Garcia, M.T.; Gonzalez-Quinonez, N.; Binda, E.; Marinelli, F.; Manteca, A.
    Characterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance, and germination in Streptomyces coelicolor (2016), Sci. Rep., 6, 21659 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.16.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
L684P about 50% of wild-type activity Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
penicillin
-
 Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane protein harbors two protein domains separated by a putative hydrophobic transmembrane region Streptomyces coelicolor 16020
-

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9KZY7
-
-
Streptomyces coelicolor ATCC BAA-471 Q9KZY7
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O
-
 Streptomyces coelicolor 2 D-Ala
-
 ?
D-Ala-D-Ala + H2O
-
 Streptomyces coelicolor ATCC BAA-471 2 D-Ala
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O substrate mimics the terminal portions of the peptidoglycan pentapeptide precursors Streptomyces coelicolor Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O substrate mimics the terminal portions of the peptidoglycan pentapeptide precursors Streptomyces coelicolor ATCC BAA-471 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?

Synonyms

Synonyms Comment Organism
DD-CPase
-
 Streptomyces coelicolor
SCO4439
-
 Streptomyces coelicolor

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
 pH 7.2, 37°C Streptomyces coelicolor penicillin

General Information

General Information Comment Organism
physiological function in a gene disruption mutant, the spores are affected in their resistance to heat and acid and show a dramatic increase in swelling during germination. The mycelium of the mutant is more sensitive to glycopeptide antibiotics vancomycin and teicoplanin. Both the DD-CPase domain and the hydrophobic transmembrane region are essential for complementing the wild type phenotypes in the mutant. In a model for the biological mechanism, DD-CPase releases D-Ala from peptidoglycan precursors, thereby reducing the substrate pool for peptidoglycan crosslinking (transpeptidation) Streptomyces coelicolor