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Literature summary for 3.4.19.5 extracted from
- beta-Aspartyl peptidase from rat liver (1970), Methods Enzymol., 19, 737-741.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight activation | Rattus norvegicus |  |
General Stability
| General Stability | Organism |
|---|---|
| stability is dependent on the presence of sodium ions | Rattus norvegicus |
| stable to freezing and thawing in 0.01 M sodium phosphate buffer | Rattus norvegicus |
| unstable to dialysis against water or 0.01 M Tris-HCl buffer, pH 8.0, if 0.01 M NaCl is included in the dialysis solution, 80% of the activity is recovered | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| cardiac muscle | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.007 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| room temperature, stable for 5 days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Asp-Gly-Ala + H2O | 55% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Gly-Ala | - |
? | |
| Asp-Gly-Val + H2O | 13% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Gly-Val | - |
? | |
| beta-Asp-Ala + H2O | 51% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Ala | - |
? | |
| beta-Asp-Gly + H2O | - |
Rattus norvegicus | Asp + Gly | - |
? | |
| beta-Asp-Gly-Gly + H2O | 95% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Gly-Gly | - |
? | |
| beta-Asp-Ile + H2O | 37% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Ile | - |
? | |
| beta-Asp-Leu + H2O | 59-65% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Leu | - |
? | |
| beta-Asp-Met + H2O | 82% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Met | - |
? | |
| beta-Asp-Ser + H2O | 47-56% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Ser | - |
? | |
| beta-Asp-Thr + H2O | 29% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Thr | - |
? | |
| beta-Asp-Val + H2O | 28% of the activity with beta-aspartylglycine | Rattus norvegicus | Asp + Val | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | Tris-HCl buffer | Rattus norvegicus |
| 7.5 | 8 | sodium phosphate buffer | Rattus norvegicus |
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Release 2023.1 (January 2023)
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