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Literature summary for 3.4.21.111 extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Engineering of S2 site of aqualysin I; alteration of P2 specificity by excluding P2 side chain (1998), Biochemistry, 37, 17402-17407.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus aquaticus

Protein Variants

Protein Variants Comment Organism
G101A catalytic efficiencies for bulky amino acid residues in P2 site such as valine and lucine drastically decreased Thermus aquaticus
G101L reduced catalytic efficiencies for any substrate Thermus aquaticus
G101V catalytic efficiency toward glycine was retained Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
0.76
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
0.91
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
1
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
1.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
1.3
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
1.5
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
1.6
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
1.7
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
3.4
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
4.2
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
5.3
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
36
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
39
-
 N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
44
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
71
-
 N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
730
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
1200
-
 N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Gly-Gly-Phe + 4-nitroaniline
-
 ?
N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Leu-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Leu-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Ala + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
0.014
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
0.024
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101L mutant Thermus aquaticus
0.091
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
0.15
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
0.17
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
0.18
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
0.18
-
 N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
0.22
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
0.25
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
0.88
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
0.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101V mutant Thermus aquaticus
2.5
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.3
-
 N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
4.7
-
 N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
6.08
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G101A mutant Thermus aquaticus
8.8
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus