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Literature summary for 3.4.21.111 extracted from

  • Matsuzawa, H.
    Aqualysin I (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , Eds. ) Academic Press, 2, 1799-1800.
No PubMed abstract available
Search for more literature for 3.4.21.111

Application

Application Comment Organism
additional information C-terminal pro sequence of the enzyme functions as an intramolecular chaperone that stabilizes the partially unfolded structure of the protease domain, and thereby facilitates its secretion Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Thermus thermophilus Thermus aquaticus

Inhibitors

Inhibitors Comment Organism Structure
DFP strongly inhibits Thermus aquaticus
N-terminal propeptide of aqualisin I potent inhibitor Thermus aquaticus
Streptomyces subtilisin inhibitor strongly inhibits Thermus aquaticus
Z-Ala-Gly-Phe-CH2Cl strongly inhibits Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizes, at least two Ca2+-binding sites, i. e. stronger and weaker binding sites, weaker binding site essential for heat stability of the enzyme, Ca2+ bound to the stronger binding site is hardly removed with EDTA Thermus aquaticus
La3+ stabilizes Thermus aquaticus
Nd3+ stabilizes Thermus aquaticus
Sr2+ stabilizes Thermus aquaticus
Tb3+ stabilizes Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28350
-
 calculated Thermus aquaticus
38000
-
 precursor with a C-terminal pro sequence from the membrane fraction of Escherichia coli cells Thermus aquaticus
53910
-
 synthesized as a large precursor, calculated Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus aquaticus YT-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli cells harboring an expression plasmid using the tac promoter by cation-exchange chromatography, larger scale production by using bacteriophage T7 RNA polymerase/promoter Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
elastin-orcein + H2O at 40°C, pH 7.5 Thermus aquaticus ?
-
 ?
elastin-orcein + H2O at 40°C, pH 7.5 Thermus aquaticus YT-1 ?
-
 ?
Hammarsten casein + H2O at 70°C, pH 7.5-10.4 Thermus aquaticus ?
-
 ?
Hammarsten casein + H2O at 70°C, pH 7.5-10.4 Thermus aquaticus YT-1 ?
-
 ?
additional information exhibits specificity toward ester of amino acids with small haydrophobic or aromatic residues in P1 Thermus aquaticus ?
-
 ?
additional information exhibits specificity toward ester of amino acids with small haydrophobic or aromatic residues in P1 Thermus aquaticus YT-1 ?
-
 ?
oxidized insulin chain B + H2O at 40°C, pH 7.5 Thermus aquaticus ?
-
 ?
oxidized insulin chain B + H2O at 40°C, pH 7.5 Thermus aquaticus YT-1 ?
-
 ?
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O at 40°C, pH 7.5 Thermus aquaticus Suc-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O at 40°C, pH 7.5 Thermus aquaticus YT-1 Suc-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 in the presence of 1 mM CaCl2, which stabilizes the enzyme Thermus aquaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80
-
 Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Thermus aquaticus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.4
-
 Thermus aquaticus