Crystallization (Comment) | Organism |
---|---|
FX99 mutant of trypsin, hanging drop vapour diffusion method, using 1.6 M ammonium sulfate | Streptomyces griseus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the FX99 mutant is constructed using 19 replacements in wild type trypsin with residues present in FXa; i.e. N95T/G96K/T97E/insertionT98/insertionY99 in the 99-loop, A171S/Y172S/G173S/N174F/E175I/E180M in the 170-loop, DELTAP185/G186K/G187Q/V188E in the 186-loop, and Y217E/P222K/Y224K/P225Y in the 220-loop | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | Na+ activation is interwoven with substrate selectivity in the trypsin scaffold | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |