Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | proteolytic activity requires presence of dithiothreitol. Autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol | Clostridioides difficile |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridioides difficile |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E64 | 0.1 mM, complete inhibition | Clostridioides difficile | |
EDTA | 5 mM, 81% residual activity | Clostridioides difficile | |
iodoacetic acid | 10 mM, 15% residual activity | Clostridioides difficile | |
leupeptin | 0.1 mM, less than 10% residual activity | Clostridioides difficile | |
N-ethylmaleimide | 10 mM, less than 10% residual activity | Clostridioides difficile | |
pepstatin | 0.01 mM, 90% residual activity | Clostridioides difficile | |
phenylmethylsulfonyl fluoride | 10 mM, less than 10% residual activity | Clostridioides difficile | |
TLCK | 10 mM, less than 10% residual activity | Clostridioides difficile |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | enzyme is found to be associated with the S-layer proteins, mostly as a nonmature species | Clostridioides difficile | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE | Clostridioides difficile |
68000 | - |
x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE | Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | Q83UF3 | - |
- |
Clostridioides difficile 79-685 | Q83UF3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol or trypsin | Clostridioides difficile |
Purification (Comment) | Organism |
---|---|
- |
Clostridioides difficile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibronectin + H2 | - |
Clostridioides difficile | ? | enzyme cleaves fibronectin to produce two bands | ? | |
fibronectin + H2 | - |
Clostridioides difficile 79-685 | ? | enzyme cleaves fibronectin to produce two bands | ? | |
laminin + H2 | - |
Clostridioides difficile | ? | - |
? | |
laminin + H2 | - |
Clostridioides difficile 79-685 | ? | - |
? | |
additional information | proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen | Clostridioides difficile | ? | - |
? | |
additional information | proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen | Clostridioides difficile 79-685 | ? | - |
? | |
vitronectin + H2 | - |
Clostridioides difficile | ? | - |
? | |
vitronectin + H2 | - |
Clostridioides difficile 79-685 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE | Clostridioides difficile |
Synonyms | Comment | Organism |
---|---|---|
Cwp84 | - |
Clostridioides difficile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Clostridioides difficile |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | more than 80% of maximum activity | Clostridioides difficile |
10 | - |
30% of maximum activity | Clostridioides difficile |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Clostridioides difficile | isoelectric focusing | - |
7.5 |