Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation of gingipain R by incubation for 10 min in 200 mM Tris-HCl buffer, pH 7.6, containing 50 mM Gly-Gly, 10 mM CaCl2 and 10 mM Cys-HCl | Porphyromonas gingivalis |
Application | Comment | Organism |
---|---|---|
medicine | development of potent, gingipain-specific inhibitors might be a helpful tool in a therapeutic strategy to prevent or treat periodontal disease | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine pancreatic secretory trypsin inhibitor | i.e. PSTI bovine, about 33% inhibition at 0.0005 mM, 66% at 0.001 mM, and 87% at 0.0025 mM, a Kazal-type serine proteinase inhibitor purified from pancreas, bovine pancreatic secretory trypsin inhibitor having an essential Arg residue at the P1 position of the reactive site, and containing Tyr and Asn residues the P2' and P3' sites, specifically inhibits the activity of the Arg-specific gingipain R, whereas porcine inhibitor, possessing a Lys residue at the P1 position, exhibits activity only against the Lys-specific cysteine proteinase gingipain K, EC 3.4.22.47. The inhibitory effect is eliminated by Arg residue modification in 0.2 M borate buffer, pH 9.0, and 50 mM excess of cyclohexanedione in a 1 : 30 molar ratio at 37°C. The association equilibrium constant is 0.0016 mM | Porphyromonas gingivalis | |
carbobenzoxy-Glu(NHN(CH3)Ph)-Lys-CO-NHCH2Ph | - |
Porphyromonas gingivalis | |
carbobenzoxy-Lys-Arg-CO-Lys-N-(CH3)2 | - |
Porphyromonas gingivalis | |
chicken ovoinhibitor | III and IV domains having Leu and Ser or Leu and Leu, respectively, at the P2' and P3' sites | Porphyromonas gingivalis | |
Chloromethylketones | - |
Porphyromonas gingivalis | |
EDTA | - |
Porphyromonas gingivalis | |
iodoacetamide | - |
Porphyromonas gingivalis | |
leupeptin | - |
Porphyromonas gingivalis | |
tetracycline | - |
Porphyromonas gingivalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Porphyromonas gingivalis | gingipains are key virulence determinants of Porphyromonas gingivalis and play a crucial role in pathogenicity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | gingipains are key virulence determinants of Porphyromonas gingivalis and play a crucial role in pathogenicity | Porphyromonas gingivalis | ? | - |
? | |
N-alpha-benzoyl-DL-arginine 4-nitroanilide + H2O | - |
Porphyromonas gingivalis | N-alpha-benzoyl-DL-arginine + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arg-specific cysteine proteinase gingipain | - |
Porphyromonas gingivalis |
gingipain R | - |
Porphyromonas gingivalis |
RGP | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.3 | assay at | Porphyromonas gingivalis |