General Stability | Organism |
---|---|
Ca2+ stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
EDTA | - |
Homo sapiens | |
additional information | not: inhibitors for serine, cysteine or aspartic proteinases; phosphoramidon | Homo sapiens | |
NEM | - |
Homo sapiens | |
U24522 | synthetic inhibitor known to inhibit proteoglycan-degrading metalloproteinases | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly-ethylester | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
expression in Escherichia coli | - |
Homo sapiens | - |
catalytic domain lacking both propeptide and C-terminal fragment | - |
Purification (Comment) | Organism |
---|---|
catalytic domain lacking both propeptide and C-terminal fragment | Homo sapiens |
expression in Escherichia coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 7.6, 10 mM CaCl2, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
Proteoglycan + H2O | - |
Homo sapiens | More | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable at pH 6-9 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.12 | - |
Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly-ethylester | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | pH 5.0: about 20% of activity maximum, pH 7.5: about 25% of activity maximum | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 37°C, stable | Homo sapiens |