Crystallization (Comment) | Organism |
---|---|
three-times crystallized preparation, Lot T5CB491 from Daiwa Kasei, Osaka | Bacillus thermoproteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zincov | competitive inhibitor | Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | active zinc ion in thermolysin can be substituted by Co2+, doubles activity | Bacillus thermoproteolyticus | |
NaBr | 4 M enhances activity 13-15 times | Bacillus thermoproteolyticus | |
NaCl | 4 M enhances activity 13-15 times | Bacillus thermoproteolyticus | |
Zn2+ | active zinc ion in thermolysin | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
commercial preparation, used without further purification | Bacillus thermoproteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
r | |
Nalpha-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Bacillus thermoproteolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | - |
Bacillus thermoproteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000012 | - |
Zincov | pH 7.5, 25°C, cobalt-substituted thermolysin | Bacillus thermoproteolyticus | |
0.00023 | - |
Zincov | pH 7.5, 25°C | Bacillus thermoproteolyticus |