KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.5, 25°C, recombinant enzyme | Bacillus thermoproteolyticus | |
0.52 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.5, 25°C, native enzyme | Bacillus thermoproteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34600 | - |
x * 34600, SDS-PAGE | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
expressed in Escherichia coli | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | synthesis as a pre-proprotein | Bacillus thermoproteolyticus |
Purification (Comment) | Organism |
---|---|
expression as single polypeptide pre-proenzyme in Escherichia coli, secretion into medium as mature enzyme | Bacillus thermoproteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9500 | - |
pH 7.5, 25°C | Bacillus thermoproteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbobenzoxy-L-Asp-L-Phe-methyl ester + H2O | - |
Bacillus thermoproteolyticus | N-carbobenzoxy-L-Asp + L-Phe-methyl ester | - |
? | |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34600, SDS-PAGE | Bacillus thermoproteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.5, 25°C, recombinant enzyme | Bacillus thermoproteolyticus | |
4.3 | - |
N-carbobenzoxy-L-Asp-L-Phe methyl ester | pH 7.5, 25°C, native enzyme | Bacillus thermoproteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Bacillus thermoproteolyticus |