Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity | Acinetobacter calcoaceticus | |
1,10-phenanthroline | Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity | Escherichia coli | |
antipain | - |
Acinetobacter calcoaceticus | |
chymostatin | - |
Acinetobacter calcoaceticus | |
Co2+ | - |
Acinetobacter calcoaceticus | |
Co2+ | - |
Escherichia coli | |
DTT | - |
Acinetobacter calcoaceticus | |
DTT | - |
Escherichia coli | |
EDTA | Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity | Acinetobacter calcoaceticus | |
EDTA | Co2+, and to a lesser extent but at lower metal ion concentration Zn2+ restores activity | Escherichia coli | |
PCMB | - |
Acinetobacter calcoaceticus | |
phosphoramidon | - |
Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000105 | - |
Insulin | - |
Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
periplasm | - |
Acinetobacter calcoaceticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | reactivation after inhibition with chelating agents | Escherichia coli | |
Co2+ | reactivation after inhibition with chelating agents | Acinetobacter calcoaceticus | |
Zn2+ | most effective metal ion in reactivation after inhibition by chelating agent | Escherichia coli | |
Zn2+ | most effective metal ion in reactivation after inhibition by chelating agent | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE | Escherichia coli |
107000 | - |
1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
similar enzyme | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glucagon + H2O | - |
Escherichia coli | ? | - |
? | |
Glucagon + H2O | - |
Acinetobacter calcoaceticus | ? | - |
? | |
Insulin + H2O | - |
Escherichia coli | Hydrolyzed insulin | - |
? | |
Insulin + H2O | - |
Acinetobacter calcoaceticus | Hydrolyzed insulin | - |
? | |
Insulin B-chain + H2O | - |
Escherichia coli | ? | - |
? | |
Insulin B-chain + H2O | cleavage sites are Asn3-Gln4, His10-Leu11, Ala14-Leu15, Leu17-Val18, Gly23-Phe24, Phe24-Phe25, Phe25-Tyr26, the enzyme shares one of the only two cleavage sites with pitrilysin and 4 sites with insulin-destroying insulinase (EC 3.4.24.56) | Acinetobacter calcoaceticus | ? | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | prefers the cleavage of small polypeptides to the cleavage of proteins, inactive against synthetic amino acid derivatives of subtilisin, thermolysin, trypsin and chymotrypsin | Acinetobacter calcoaceticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE | Escherichia coli |
monomer | 1 * 107000, Acinetobacter calcoaceticus, SDS-PAGE | Acinetobacter calcoaceticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
45 | - |
- |
Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.2 | insulin | Acinetobacter calcoaceticus |