Cloned (Comment) | Organism |
---|---|
rat, testis | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.1 mM | Rattus norvegicus | |
amastatin | 0.02 mM | Rattus norvegicus | |
bestatin | 0.02 mM | Rattus norvegicus | |
Ca2+ | not | Rattus norvegicus | |
Co2+ | 0.1 mM | Rattus norvegicus | |
Cu2+ | 0.1 mM | Rattus norvegicus | |
DTT | weak | Rattus norvegicus | |
EDTA | 0.01 mM; not by Cu2+; reversible by low concentrations of Zn2+ or Mn2+; to a lesser extent by Co2+, Ca2+; to a lesser extent by Mg2+ (not) | Rattus norvegicus | |
EGTA | 0.01 mM | Rattus norvegicus | |
Mn2+ | - |
Rattus norvegicus | |
additional information | Mg2+ (0.1 mM); no inhibition by IAA, phosphoramidon, captopril, phenylmethylsulfonyl fluoride, leupeptin, guanidylethylmercaptosuccinic acid (i.e. GEMSA) | Rattus norvegicus | |
N-ethylmaleimide | - |
Rattus norvegicus | |
N-tosyl-L-Phe chloromethyl ketone | - |
Rattus norvegicus | |
Zn2+ | 0.1 mM; strong | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00625 | - |
ANF-(1-28) | - |
Rattus norvegicus | |
0.00645 | - |
dynorphin A | - |
Rattus norvegicus | |
0.0173 | - |
Preproneurotensin-(154-170) | - |
Rattus norvegicus | |
0.024 | - |
dynorphin A-(1-13) | - |
Rattus norvegicus | |
0.043 | - |
Somatostatin-28 | - |
Rattus norvegicus | |
0.103 | - |
Bovine adrenal medulla dodecapeptide | - |
Rattus norvegicus | |
0.127 | - |
dynorphin A-(1-10) | - |
Rattus norvegicus | |
0.162 | - |
dynorphin A-(1-9) | - |
Rattus norvegicus | |
0.209 | - |
dynorphin B | - |
Rattus norvegicus | |
0.227 | - |
[Arg0,Ala17]-somatostatin-28-(1-19)-NH2 | - |
Rattus norvegicus | |
0.428 | - |
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 | or peptide I | Rattus norvegicus | |
0.974 | - |
alpha-neoendorphin | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Rattus norvegicus |
90000 | - |
rat, brain | Rattus norvegicus |
110000 | - |
PAGE, 2 catalytically active forms: MW 110000 and MW 140000 | Rattus norvegicus |
110000 | - |
rat, testis | Rattus norvegicus |
133000 | - |
rat, testis, deduced from nucleotide sequence | Rattus norvegicus |
140000 | - |
PAGE, 2 catalytically active forms: MW 110000 and MW 140000 | Rattus norvegicus |
140000 | - |
rat, testis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
hypophysis | - |
Rattus norvegicus | - |
hypothalamus | - |
Rattus norvegicus | - |
additional information | tissue distribution | Rattus norvegicus | - |
neocortex | - |
Rattus norvegicus | - |
testis | predominantly | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-Neoendorphin + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Leu, cleavage at Arg-Lys processing site | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu-Arg + Lys-Tyr-Pro-Leu | - |
? | |
ANF-(1-28) + H2O | i.e. atrial natriuretic factor, cleavage site: Arg+Arg | Rattus norvegicus | ? | - |
? | |
Bovine adrenal medulla dodecapeptide + H2O | i.e. Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu | Rattus norvegicus | Tyr-Gly-Gly-Phe-Met-Arg + Arg-Val-Gly-Arg-Pro-Glu | - |
? | |
dynorphin A + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln-NH2 | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln-NH2 | - |
? | |
dynorphin A-(1-10) + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro, cleavage site: Arg-Arg | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro | - |
? | |
dynorphin A-(1-13) + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys, cleavage site: Arg-Arg | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys | - |
? | |
dynorphin A-(1-9) + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg, cleavage site: Arg-Arg | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg | - |
? | |
dynorphin B + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Gln-Phe-Lys-Val-Val-Thr | - |
? | |
additional information | [Tyr20]-somatostatin-28-(13-20)-NH2, dynorphin A-(1-7), [Ala17]-somatostatin-(6-19)-NH2, prosomatostatin-(56-68)-NH2, the Lys-Arg-doublet in preproenkephalin-(128-140) or pro-oxytocin/neurophysin-(1-20) | Rattus norvegicus | ? | - |
? | |
additional information | cleavage occurs selectively at amino-terminal side of an Arg-residue in dibasic stretches | Rattus norvegicus | ? | - |
? | |
Preproneurotensin-(154-170) + H2O | cleavage site: prohormonal maturation site, Lys-Arg | Rattus norvegicus | ? | - |
? | |
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O | i.e. model peptide I | Rattus norvegicus | Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2 | - |
? | |
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O | i.e. Pro-Arg-Glu-Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2 | Rattus norvegicus | Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2 | - |
? | |
[Arg0,Ala17]-somatostatin-28-(1-19)-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 110000, testis, SDS-PAGE, 1 * 140000, brain, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
peptide I as substrate | Rattus norvegicus |