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Literature summary for 3.4.99.B1 extracted from

  • Dolence, J.M.; Steward, L.E.; Dolence, E.K.; Wong, D.H.; Poulter, C.D.
    Studies with Recombinant Saccharomyces cerevisiae CaaX Prenyl Protease Rce1p (2000), Biochemistry, 39, 4096-4104.
    View publication on PubMed
Search for more literature for 3.4.99.B1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C251A no proteolytic activity Saccharomyces cerevisiae
E156A no proteolytic activity Saccharomyces cerevisiae
H194A no proteolytic activity Saccharomyces cerevisiae
H196A 50% of wild-type activity Saccharomyces cerevisiae
H197A 8% of wild-type activity Saccharomyces cerevisiae
H248A no proteolytic activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 0.087 mM, 97% inhibition Saccharomyces cerevisiae
Co2+ 0.087 mM, 48% inhibition Saccharomyces cerevisiae
Cu+ 0.087 mM, 97% inhibition Saccharomyces cerevisiae
Mersalyl acid 0.022 mM, 99% inhibition Saccharomyces cerevisiae
methyl methanothiosulfonate 0.05 mM, 40% inhibition Saccharomyces cerevisiae
additional information not inhibited by protese inhibitors bestatin, antipain, chymostatin, pepstatin A, leupeptin, E64 and PMSF Saccharomyces cerevisiae
N-Boc-Cys(farnesyl)-methylenamin-VIM-OH 0.0001 mM, 50% inhibition Saccharomyces cerevisiae
Nalpha-tosyl-L-lysine chloromethyl ketone 0.3 mM, 32% inhibition Saccharomyces cerevisiae
Nalpha-tosyl-L-phenylalanine chloromethyl ketone 0.3 mM, 94% inhibition Saccharomyces cerevisiae
Ni2+ 0.087 mM, 48% inhibition Saccharomyces cerevisiae
p-hydroxymercuribenzoic acid 0.022 mM, 99% inhibition Saccharomyces cerevisiae
p-Hydroxymercuriphenylsulfonic acid 0.022 mM, 99% inhibition Saccharomyces cerevisiae
Zn2+ 0.087 mM, 99% inhibition Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
integral to membrane
-
 Saccharomyces cerevisiae
-
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-G(farnesyl)CIIS + H2O
-
 Saccharomyces cerevisiae dansyl-G(farnesyl)C + IIS
-
 ?
dansyl-GLP(farnesyl)CVVM + H2O
-
 Saccharomyces cerevisiae dansyl-GLP(farnesyl)C + VVM
-
 ?
dansyl-KTK(farnesyl)CVIM + H2O
-
 Saccharomyces cerevisiae dansyl-KTK(farnesyl)C + VIM
-
 ?
dansyl-SAK(farnesyl)CVLS + H2O
-
 Saccharomyces cerevisiae dansyl-SAK(farnesyl)C + VLS
-
 ?
dansyl-WDPA(farnesyl)CVIA + H2O
-
 Saccharomyces cerevisiae dansyl-WDPA(farnesyl)C + VIA
-
 ?
dansyl-WDPA(geranylgeranyl)CVIA + H2O
-
 Saccharomyces cerevisiae dansyl-WDPA(geranylgeranyl)C + VIA
-
 ?

Synonyms

Synonyms Comment Organism
Ras and a-factor converting enzyme
-
 Saccharomyces cerevisiae
Rce1p
-
 Saccharomyces cerevisiae