Cloned (Comment) | Organism |
---|---|
the gene bc1960 is cloned and expressed in Escherichia coli | Bacillus cereus |
the gene bc3618 is cloned and expressed in Escherichia coli | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | - |
Bacillus cereus | |
ZnCl2 | - |
Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
0.37 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
0.45 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
0.5 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
1.18 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
1.5 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
2.2 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
2.46 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus | |
3.9 | - |
GlcNAc-beta-1,4-GlcNAc | - |
Bacillus cereus | |
4.1 | - |
GlcNAc-beta-1,4-GlcNAc | enzyme BC1960 | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 10% increase in activity | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated peptidoglycan + H2O | Bacillus cereus | enzymatic deacetylation of chemically acetylated vegetative peptidoglycan from Bacillus cereus by BC1960 and BC3618 results in increased resistance to lysozyme digestion | deacetylated peptidoglycan + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated peptidoglycan + H2O | enzymatic deacetylation of chemically acetylated vegetative peptidoglycan from Bacillus cereus by BC1960 and BC3618 results in increased resistance to lysozyme digestion | Bacillus cereus | deacetylated peptidoglycan + acetate | - |
? | |
acetylated peptidoglycan + H2O | effective in deacetylating cell wall peptidoglycan from the Gram(+) Bacillus cereus and Bacillus subtilis and the Gram(-) Helicobacter pylori | Bacillus cereus | deacetylated peptidoglycan + acetate | - |
? | |
GlcNAc-beta-1,4-GlcNAc + H2O | no deacetylation of the reducing GlcNAc residue | Bacillus cereus | ? | - |
? | |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc is the favorable substrate. Deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | deacetylates all GlcNAc residues of the oligomer except the reducing end ones | Bacillus cereus | ? | - |
? | |
additional information | no activity with N-acetyl-D-glucosamine | Bacillus cereus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enzyme BC1960 | - |
Bacillus cereus |
enzyme BC3618 | - |
Bacillus cereus |
peptidoglycan N-acetylglucosamine deacetylase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme BC3618 | Bacillus cereus |
50 | - |
enzyme BC1960 | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme BC1960 retains 95% of its activity after 24 h | Bacillus cereus |
50 | - |
enzyme BC3618 is inactivated after 1h | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
enzyme BC1960 | Bacillus cereus |
8 | - |
enzyme BC3618 | Bacillus cereus |