Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | effectively reverses heavy metal inactivation | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.00125 mM, 100% inhibition | Pseudomonas putida | |
arsenite | characteristic of enzymes with essential vicinal sulfhydryl groups | Pseudomonas putida | |
Ba2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
Ca2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
Cd(acetate)2 | 0.00125 mM, 94% inhibition | Pseudomonas putida | |
Cd2+ | 0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups | Pseudomonas putida | |
Co2+ | 5.0 mM,75% inhibition | Pseudomonas putida | |
Cu2+ | 5.0 mM, complete inhibition | Pseudomonas putida | |
Fe2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
Hg(NO3)2 | 0.187 mM, 100% inhibition | Pseudomonas putida | |
Hg2+ | 0.5 mM, complete inhibition | Pseudomonas putida | |
HgCl2 | 0.00125 mM, 100% inhibition | Pseudomonas putida | |
Mg2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
NaAsO2 | 0.0125 mM, 47% inhibition | Pseudomonas putida | |
p-hydroxymercuribenzoate | 0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol | Pseudomonas putida | |
Pb2+ | 5.0 mM, complete inhibition | Pseudomonas putida | |
Sn2+ | 5.0 mM, complete inhibition | Pseudomonas putida | |
Zn2+ | 0.5 mM, complete inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
6-Aminohexanamide | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 0.125 mM stimulates activity 37% | Pseudomonas putida | |
Fe3+ | 0.125 mM stimulates activity 25% | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminopentanamide + H2O | Pseudomonas putida | known intermediate in lysine catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified 400fold from the soluble fraction of cell-free extract | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminopentanamide + H2O | - |
Pseudomonas putida | 5-aminopentanoate + NH3 | - |
? | |
5-aminopentanamide + H2O | known intermediate in lysine catabolism | Pseudomonas putida | ? | - |
? | |
6-aminohexanamide + H2O | - |
Pseudomonas putida | 6-aminohexanoate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
delta-aminovaleramidase | - |
Pseudomonas putida |
delta-aminovaleramide amidohydrolase | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | stable below pH 6.5, irreversibly inactivated above pH 9 | Pseudomonas putida |