Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM: complete inhibition | Soil bacterium | |
iodoacetate | 1 mM: strong inhibition | Soil bacterium | |
p-chloromercuribenzoate | 1 mM: strong inhibition | Soil bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for activity, optimum concentration about 1 mM | Soil bacterium | |
divalent cations | required for activity | Soil bacterium | |
Zn2+ | required for activity, optimum concentration about 1 mM | Soil bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
strain F-96, x * 39000, SDS-PAGE | Soil bacterium |
116000 | - |
strain F-96, gel filtration | Soil bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Soil bacterium | - |
- |
- |
Soil bacterium F-96 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain F-96 | Soil bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity | Soil bacterium | ? | - |
? | |
additional information | hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids | Soil bacterium | ? | - |
? | |
additional information | broadly selective amino acid specificity | Soil bacterium | ? | - |
? | |
additional information | specific for L-isomers | Soil bacterium | ? | - |
? | |
additional information | no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein | Soil bacterium | ? | - |
? | |
additional information | inactive toward Nalpha-benzyloxycarbonyl-D-amino acids | Soil bacterium | ? | - |
? | |
additional information | requires substrates with free carboxyl group of amino acids | Soil bacterium | ? | - |
? | |
additional information | substrate specificity | Soil bacterium F-96 | ? | - |
? | |
additional information | hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids | Soil bacterium F-96 | ? | - |
? | |
additional information | broadly selective amino acid specificity | Soil bacterium F-96 | ? | - |
? | |
additional information | specific for L-isomers | Soil bacterium F-96 | ? | - |
? | |
additional information | no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein | Soil bacterium F-96 | ? | - |
? | |
Nalpha-(4-aminobenzoyl)-L-Glu + H2O | poor substrate | Soil bacterium | 1-amino-4-hydroxybenzene + CO2 + L-Glu | - |
? | |
Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O | poor substrate | Soil bacterium | 1,4-dihydroxybenzene + CO2 + L-Asp | - |
? | |
Nalpha-acetyl-L-Asp + H2O | poor substrate | Soil bacterium | methanol + CO2 + L-Asp | - |
? | |
Nalpha-acetyl-L-Glu + H2O | poor substrate | Soil bacterium | methanol + CO2 + L-Glu | - |
? | |
Nalpha-acetyl-L-Leu + H2O | 40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis | Soil bacterium | methanol + CO2 + L-Leu | - |
? | |
Nalpha-benzoyl-L-Glu + H2O | - |
Soil bacterium | phenol + CO2 + L-Glu | - |
? | |
Nalpha-benzoyl-L-Leu + H2O | - |
Soil bacterium | phenol + CO2 + L-Leu | - |
? | |
Nalpha-benzyloxycarbonyl-Gly + H2O | poor substrate | Soil bacterium | benzyl alcohol + CO2 + Gly | - |
? | |
Nalpha-benzyloxycarbonyl-L-Ala + H2O | - |
Soil bacterium | benzyl alcohol + CO2 + L-Ala | - |
? | |
Nalpha-benzyloxycarbonyl-L-Asn + H2O | poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Asn | - |
? | |
Nalpha-benzyloxycarbonyl-L-Asp + H2O | - |
Soil bacterium | benzyl alcohol + CO2 + L-Asp | - |
? | |
Nalpha-benzyloxycarbonyl-L-Cys + H2O | poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Cys | - |
? | |
Nalpha-benzyloxycarbonyl-L-Gln + H2O | poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Gln | - |
? | |
Nalpha-benzyloxycarbonyl-L-Glu + H2O | - |
Soil bacterium | benzyl alcohol + CO2 + L-Glu | - |
? | |
Nalpha-benzyloxycarbonyl-L-His + H2O | very poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-His | - |
? | |
Nalpha-benzyloxycarbonyl-L-Ile + H2O | very poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Ile | - |
? | |
Nalpha-benzyloxycarbonyl-L-Met + H2O | - |
Soil bacterium | benzyl alcohol + CO2 + L-Met | - |
? | |
Nalpha-benzyloxycarbonyl-L-Phe + H2O | very poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Phe | - |
? | |
Nalpha-benzyloxycarbonyl-L-Ser + H2O | poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Ser | - |
? | |
Nalpha-benzyloxycarbonyl-L-Thr + H2O | very poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Thr | - |
? | |
Nalpha-benzyloxycarbonyl-L-Tyr + H2O | very poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Tyr | - |
? | |
Nalpha-benzyloxycarbonyl-L-Val + H2O | poor substrate | Soil bacterium | benzyl alcohol + CO2 + L-Val | - |
? | |
Nalpha-benzyloxycarbonyl-Leu + H2O | best substrate | Soil bacterium | benzyl alcohol + CO2 + L-Leu | - |
? | |
Nalpha-tert-butoxycarbonyl-Gly + H2O | - |
Soil bacterium | tert-butanol + CO2 + Gly | - |
? | |
Nalpha-tert-butoxycarbonyl-L-Ala + H2O | - |
Soil bacterium | tert-butanol + CO2 + L-Ala | - |
? | |
Nalpha-tert-butoxycarbonyl-L-Asp + H2O | - |
Soil bacterium | tert-butanol + CO2 + L-Asp | - |
? | |
Nalpha-tert-butoxycarbonyl-L-Gln + H2O | poor substrate | Soil bacterium | tert-butanol + CO2 + L-Gln | - |
? | |
Nalpha-tert-butoxycarbonyl-L-Leu + H2O | 80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis | Soil bacterium | tert-butanol + CO2 + L-Leu | - |
? | |
Nalpha-tert-butoxycarbonyl-L-Met + H2O | poor substrate | Soil bacterium | tert-butanol + CO2 + L-Met | - |
? | |
Nalpha-tert-butoxycarbonyl-L-Val + H2O | poor substrate | Soil bacterium | tert-butanol + CO2 + L-Val | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | strain F-96, x * 39000, SDS-PAGE | Soil bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Soil bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
17 h, pH 6-7, stable at | Soil bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 3.31 | Soil bacterium |
5.1 | - |
- |
Soil bacterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 17 h, 37°C, stable at | Soil bacterium |