Cloned (Comment) | Organism |
---|---|
cloned into pBC-SK and expressed in Escherichia coli HB101 | Klebsiella aerogenes |
Protein Variants | Comment | Organism |
---|---|---|
E104K | side directed mutagenesis | Klebsiella aerogenes |
E240V | side directed mutagenesis | Klebsiella aerogenes |
E240V | substitution increases catalytic efficiency toward ceftazidime | Klebsiella aerogenes |
M182T | side directed mutagenesis | Klebsiella aerogenes |
R164S | side directed mutagenesis | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanic acid | - |
Klebsiella aerogenes | |
Sulbactam | - |
Klebsiella aerogenes | |
tazobactam | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
benzylpenicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.0007 | - |
benzylpenicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.0014 | - |
piperacillin | 100 microM nitrocefin as the reporter substrate, TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.0034 | - |
piperacillin | 100 microM nitrocefin as the reporter substrate, TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.005 | - |
ampicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.016 | - |
cefepime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.019 | - |
ampicillin | 100 microM nitrocefin as the reporter substrate, TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.019 | - |
ceftazidime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.02 | - |
nitrocefin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.025 | - |
nitrocefin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.026 | - |
ceftazidime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.027 | - |
aztreonam | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.03 | - |
cefepime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.032 | - |
cefotaxime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.035 | - |
cefazolin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.043 | - |
cefotaxime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.051 | - |
aztreonam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.068 | - |
cefazolin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28600 | - |
- |
Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | Q1KY81 | SS-13 | - |
Klebsiella aerogenes SS-13 | Q1KY81 | SS-13 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isolated from Homo sapiens urine | Klebsiella aerogenes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Klebsiella aerogenes | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Klebsiella aerogenes SS-13 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
aztreonam + H2O | - |
Klebsiella aerogenes | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
aztreonam + H2O | - |
Klebsiella aerogenes SS-13 | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
benzylpenicillin + H2O | - |
Klebsiella aerogenes | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Klebsiella aerogenes SS-13 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefazolin + H2O | - |
Klebsiella aerogenes | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefazolin + H2O | - |
Klebsiella aerogenes SS-13 | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefepime + H2O | - |
Klebsiella aerogenes | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefotaxime + H2O | - |
Klebsiella aerogenes | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Klebsiella aerogenes SS-13 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | preferred and best substrate | Klebsiella aerogenes | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
nitrocefin + H2O | - |
Klebsiella aerogenes | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Klebsiella aerogenes | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-lactamase | - |
Klebsiella aerogenes |
TEM-149 | - |
Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | thermal stability of TEM-149 wild-type and TEM-149T182M mutant is determined by measuring the residual activity after incubation of the enzymes at three different temperatures: 25°C, 40°C, and 55°C. TEM-149 wild-type exhibits half-lives of 90 min at 25°C, 70 min at 40°C, and 30 min at 55°C, whereas TEM-149T182M mutant shows half-lives greater than 120 min at 25°C and 40°C and 45 min at 55°C. Compared to the thermal stability of TEM-149, the thermal stability of the TEM-149T182M mutant is substantially increased | Klebsiella aerogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
cefotaxime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.41 | - |
cefotaxime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.43 | - |
cefepime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
0.56 | - |
cefazolin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
2.2 | - |
cefazolin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
2.4 | - |
ampicillin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
2.4 | - |
cefepime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
2.7 | - |
piperacillin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.5 | - |
benzylpenicillin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
3.6 | - |
aztreonam | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
4.7 | - |
aztreonam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
5 | - |
piperacillin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
5.4 | - |
ampicillin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
7.4 | - |
nitrocefin | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
8.3 | - |
ceftazidime | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
8.3 | - |
ceftazidime | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
10.5 | - |
benzylpenicillin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes | |
26 | - |
nitrocefin | TEM-149 mutant T182M, pH 7.0, 25°C | Klebsiella aerogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000018 | - |
tazobactam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.000026 | - |
tazobactam | TEM-149T182M mutant, pH 7.0, 25°C | Klebsiella aerogenes | |
0.00011 | - |
clavulanic acid | TEM-149T182M mutant, pH 7.0, 25°C | Klebsiella aerogenes | |
0.00015 | - |
Sulbactam | TEM-149T182M mutant, pH 7.0, 25°C | Klebsiella aerogenes | |
0.00021 | - |
Sulbactam | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes | |
0.00026 | - |
clavulanic acid | TEM-149 wild-type, pH 7.0, 25°C | Klebsiella aerogenes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Klebsiella aerogenes | and above 9.0, isoelectric focusing analysis | - |
5.8 |
Klebsiella aerogenes | above, and at 5.8, isoelectric focusing analysis | - |
9 |