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Literature summary for 3.7.1.1 extracted from

  • Ruijter, G.J.G.; van de Vondervoort, P.J.I.; Visser, J.
    Oxalic acid production by Aspergillus niger: an oxalate-non-producing mutant produces citric acid at pH 5 and in the presence of manganese (1999), Microbiology, 145, 2569-2576.
No PubMed abstract available
Search for more literature for 3.7.1.1

Inhibitors

Inhibitors Comment Organism Structure
oxalate competitive inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 oxaloacetate
-
 Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Aspergillus niger 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ maximal activity at 0.2 mM Mn2+ Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + H2O Aspergillus niger
-
 oxalate + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Phenyl Sepharose, Q-Sepharose Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58
-
 partially purified enzyme Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + H2O
-
 Aspergillus niger oxalate + acetate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 in imidazol buffer Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5 9 in MES, piperazine-N,N'-bis(2-ethansulfonic acid), Tris and triethanolamine Aspergillus niger