Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyruvate | allosteric substrate activation, kinetics of dimeric and tetrameric enzyme | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
X-ray crystallography structure | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor, tetramer binds 4 Mg2+ ions, tightly bound at pH 6, dissociates reversibly above pH 7 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, catalytically inactive enzyme state, SDS-PAGE | Saccharomyces cerevisiae |
60000 | - |
2 * 60000, smallest enzymatically active unit, PDC consists of dimers and tetramers under physiological conditions, subunit interactions, SDS-PAGE | Saccharomyces cerevisiae |
60000 | - |
4 * 60000, native, active enzyme state, dimer of dimers, PDC consists of dimers and tetramers under physiological conditions, subunit interactions, SDS-PAGE | Saccharomyces cerevisiae |
240000 | - |
tetramer | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Saccharomyces cerevisiae | enzyme within the glycolytic pathway in fermenting cells | acetaldehyde + CO2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
urea | treatment with 0.5 M urea results in dimeric, with 2 M urea in monomeric enzyme state | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no difference in specific activity of dimeric and tetrameric enzyme state | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | enzyme within the glycolytic pathway in fermenting cells | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, smallest enzymatically active unit, PDC consists of dimers and tetramers under physiological conditions, subunit interactions, SDS-PAGE | Saccharomyces cerevisiae |
homotetramer | 4 * 60000, native, active enzyme state, dimer of dimers, PDC consists of dimers and tetramers under physiological conditions, subunit interactions, SDS-PAGE | Saccharomyces cerevisiae |
monomer | 1 * 60000, catalytically inactive enzyme state, SDS-PAGE | Saccharomyces cerevisiae |
More | treatment with 0.5 M urea results in dimeric, with 2 M urea in monomeric enzyme state | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PDC | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
dimer and tetramer | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requirement, tetramer binds 4 molecules, at the interface between two monomers involving the alpha and gamma domains, tightly bound at pH 6, dissociates reversibly above pH 7 | Saccharomyces cerevisiae |