Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain C43(DE3) | Vibrio cholerae serotype O1 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method using 0.1 M sodium cacodylate (pH 6.5), 0.2 M (NH4)2SO4, 5% (v/v) glycerol and 25% (w/v) PEG 8000 | Vibrio cholerae serotype O1 |
Protein Variants | Comment | Organism |
---|---|---|
C148A | 74% activity of the wild type enzyme | Vibrio cholerae serotype O1 |
C148S | 54% activity of the wild type enzyme | Vibrio cholerae serotype O1 |
D17A | inactive | Vibrio cholerae serotype O1 |
H207I | inactive | Vibrio cholerae serotype O1 |
H209I | inactive | Vibrio cholerae serotype O1 |
K178I | inactive | Vibrio cholerae serotype O1 |
K178R | inactive | Vibrio cholerae serotype O1 |
M180I | 16% activity of the wild type enzyme | Vibrio cholerae serotype O1 |
Q20L | inactive | Vibrio cholerae serotype O1 |
R16I | inactive | Vibrio cholerae serotype O1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Vibrio cholerae serotype O1 | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | active site metal ion, 2 mol Zn2+ per mol enzyme | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, gel filtration | Vibrio cholerae serotype O1 |
98300 | - |
static light scattering | Vibrio cholerae serotype O1 |
102000 | - |
gel filtration | Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA-agarose column chromatography | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxaloacetate | - |
Vibrio cholerae serotype O1 | Pyruvate + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 51000, gel filtration | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
oxaloacetate carboxylase Na+ pump | membrane-bound multiprotein complex that couples oxaloacetate decarboxylation to sodium ion transport across the membrane | Vibrio cholerae serotype O1 |