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Literature summary for 4.1.1.15 extracted from

  • Kanwal, S.; Incharoensakdi, A.
    Characterization of glutamate decarboxylase from Synechocystis sp. PCC6803 and its role in nitrogen metabolism (2016), Plant Physiol. Biochem., 99, 59-65 .
    View publication on PubMed
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene gad, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression in Escherichia coli strain BL21(DE3), real-time PCR enzyme expression analysis Synechocystis sp. PCC 6803

Protein Variants

Protein Variants Comment Organism
additional information generation of an enzyme deletion strain, DELTAgad, transcription levels of genes involved in nitrogen metabolism are upregulated in the mutant strain Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19.6
-
 L-glutamate pH 6.0, 30°C, recombinant enzyme Synechocystis sp. PCC 6803

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 recombinant enzyme, gel filtration Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Synechocystis sp. PCC 6803
-
 4-aminobutanoate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 P73043
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Synechocystis sp. PCC 6803 4-aminobutanoate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, recombinant enzyme, SDS-PAGE Synechocystis sp. PCC 6803

Synonyms

Synonyms Comment Organism
GAD
-
 Synechocystis sp. PCC 6803
sll1641 ORF name Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Synechocystis sp. PCC 6803

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 activity range, recombinant enzyme, profile overview Synechocystis sp. PCC 6803

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100.7
-
 L-glutamate pH 6.0, 30°C, recombinant enzyme Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6 recombinant enzyme Synechocystis sp. PCC 6803

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 narrow activity range, recombinant enzyme, profile overview Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on, best at 0.04 mM Synechocystis sp. PCC 6803

pI Value

Organism Comment pI Value Maximum pI Value
Synechocystis sp. PCC 6803 sequence calculation
-
 5.1

General Information

General Information Comment Organism
malfunction transcription levels of genes involved in nitrogen metabolism are upregulated in the DELTAgad enzyme deletion strain. The mutant shows approximately 4 and 8fold increases in the transcript levels of kgd and gabdh encoding a novel 2-oxoglutarate decarboxylase and gamma-aminobutanal dehydrogenase, respectively. Phenotype, overview. In Synechocystis lacking a functional GAD, the gamma-aminobutanal dehydrogenase might serve as an alternative catalytic pathway for GABA synthesis Synechocystis sp. PCC 6803
physiological function glutamate decarboxylase (GAD) is the enzyme responsible for the synthesis of gamma-aminobutyric acid (GABA) in Synechocystis sp. PCC6803 Synechocystis sp. PCC 6803

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1
-
 L-glutamate pH 6.0, 30°C, recombinant enzyme Synechocystis sp. PCC 6803