Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glycerate | product inhibition, 10 mM, 65% inhibition | Pseudomonas sp. | |
D-malate | 10 mM, 83% inhibition | Pseudomonas sp. | |
EDTA | 5 mM, at 30°C, complete inactivation | Pseudomonas sp. | |
L-Glycerate | 10 mM, 63% inhibition | Pseudomonas sp. | |
L-malate | 10 mM, 29% inhibition | Pseudomonas sp. | |
meso-tartrate | 10 mM, 14% inhibition | Pseudomonas sp. | |
succinate | 10 mM, 28% inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
NAD+ | pH 7.5, 30°C | Pseudomonas sp. | |
7 | - |
L-Tartrate | pH 7.5, 30°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Pseudomonas sp. | |
Cu2+ | slight activation | Pseudomonas sp. | |
K+ | activation, above 40 mM | Pseudomonas sp. | |
Mg2+ | activation | Pseudomonas sp. | |
Mg2+ | above 0.2 mM | Pseudomonas sp. | |
Mn2+ | activation | Pseudomonas sp. | |
additional information | requirement of a divalent and a monovalent cation, not activated by Ca2+, Fe2+, Zn2+, Li+ or Na+ | Pseudomonas sp. | |
NH4+ | slight activation | Pseudomonas sp. | |
Ni2+ | slight activation | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Pseudomonas sp. |
148000 | - |
HPLC gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tartrate | Pseudomonas sp. | inducible by L-tartrate | D-glycerate + CO2 | - |
? | |
L-tartrate | Pseudomonas sp. 5D1A | inducible by L-tartrate | D-glycerate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
soil isolate | - |
Pseudomonas sp. | - |
group Ve-2 | - |
Pseudomonas sp. 5D1A | - |
group Ve-2 | - |
Purification (Comment) | Organism |
---|---|
about 11fold | Pseudomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R,R)-tartrate = D-glycerate + CO2 | reduction proceeds concomitantly with decarboxylation, but at a much lower rate | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
pH 7.5, 30°C | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 3 months, stable, without glycerol, 1 month, 40% loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-malate + NAD+ | - |
Pseudomonas sp. | pyruvate + CO2 + NADH | - |
? | |
D-malate + NAD+ | - |
Pseudomonas sp. 5D1A | pyruvate + CO2 + NADH | - |
? | |
L-tartrate | best substrate | Pseudomonas sp. | D-glycerate + CO2 | - |
? | |
L-tartrate | inducible by L-tartrate | Pseudomonas sp. | D-glycerate + CO2 | - |
? | |
L-tartrate | best substrate | Pseudomonas sp. 5D1A | D-glycerate + CO2 | - |
? | |
L-tartrate | inducible by L-tartrate | Pseudomonas sp. 5D1A | D-glycerate + CO2 | - |
? | |
meso-tartrate | - |
Pseudomonas sp. | D-glycerate + CO2 | - |
? | |
additional information | not: dihydroxyfumarate | Pseudomonas sp. | ? | - |
? | |
additional information | enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity | Pseudomonas sp. | ? | - |
? | |
additional information | not: dihydroxyfumarate | Pseudomonas sp. 5D1A | ? | - |
? | |
additional information | enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity | Pseudomonas sp. 5D1A | ? | - |
? | |
oxaloacetate + NAD+ | - |
Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas sp. |
40 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, stable | Pseudomonas sp. |
60 | - |
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 5% loss of activity, inactivation at pH 6.5 and 9.5 | Pseudomonas sp. |
65 | - |
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 29% loss of activity | Pseudomonas sp. |
70 | - |
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, inactivation | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Pseudomonas sp. |
7.5 | - |
assay at | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | half-maximal activity at pH 5.5 and 9 | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
30 min at 60°C, inactivation | Pseudomonas sp. |
7 | - |
30 min at 60°C, 23% loss of activity | Pseudomonas sp. |
7.5 | - |
30 min at 60°C, in 20 mM Tris-HCl buffer, 0.2 mM MgCl2, 5% loss of activity | Pseudomonas sp. |
8 | - |
30 min at 60°C, 13% loss of activity | Pseudomonas sp. |
9 | - |
30 min at 60°C, 57% loss of activity | Pseudomonas sp. |
9.5 | - |
30 min at 60°C, inactivation | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | requirement | Pseudomonas sp. | |
NAD+ | decarboxylation is not stoichiometrically related with NAD+, NAD+-reduction at 1.1% the rate of L-tartrate decarboxylation | Pseudomonas sp. | |
NADP+ | requirement, less effective than NAD+ | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
4.9 |