Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
crystallized in the presence of glycerol and PEG 4000 at 4°C, producing crystals that are cryoprotected with a cryosolution containing reservoir and glycerol at a final concentration of 35% (v/v) before flash-cooling in liquid nitrogen. Crystallized in space group P22(1)2(1), with the biologically relevant tetramer in the asymmetric unit, and the structure has been determined via molecular replacement to a resolution of 2.0 A | Toxoplasma gondii |
sitting drop vapor diffusion method, using 0.1 M MOPS/HEPES-Na pH 7.9, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate, 0.02 M ammonium acetate, 12.5% (v/v) glycerol, 25% (w/v) PEG 4000 | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Toxoplasma gondii | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | Toxoplasma gondii ATCC 50861 | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | B9PW35 | - |
- |
Toxoplasma gondii ATCC 50861 | B9PW35 | - |
- |
Purification (Comment) | Organism |
---|---|
Co-TALON column chromatography and Superdex 200 gel filtration | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Toxoplasma gondii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | - |
Toxoplasma gondii ATCC 50861 | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fructose-1,6-bisphosphate aldolase | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
physiological function | during invasion of the apicomplexan parasite Toxoplasma gondii into host cells, the enzyme serves in the role of a structural bridging protein, as opposed to its normal enzymatic role in the glycolysis pathway | Toxoplasma gondii |