Cloned (Comment) | Organism |
---|---|
gene Aldob, recombinant expression of GST-tagged liver enzyme in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | mainly | Rattus norvegicus | 5737 | - |
additional information | liver fructose 1,6-bisphosphatase (FBPase-1) guides the cellular localization of aldolase B | Rattus norvegicus | - |
- |
nucleus | - |
Rattus norvegicus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Rattus norvegicus |
38000 | - |
gel filtration, recombinant enzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Rattus norvegicus | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Rattus norvegicus Wistar | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P00884 | - |
- |
Rattus norvegicus Wistar | P00884 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
recombinant GST-tagged liver enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
liver | aldolase B is the predominant isozyme in liver and is also expressed in kidney | Rattus norvegicus | - |
additional information | coexpression of fructose 1,6-bisphosphate aldolase B and fructose 1,6-bisphosphatase (FBPase-1) in cultured cells | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Rattus norvegicus | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Rattus norvegicus Wistar | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
Aldob | - |
Rattus norvegicus |
aldolase B | - |
Rattus norvegicus |
F1,6-P2 aldolase | - |
Rattus norvegicus |
fructose-1,6-bisphosphate aldolase B | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
drug target | the enzyme (FBPase-1) is considered to be a new target for the control of diabetes | Rattus norvegicus |
metabolism | metabolic conditions modulate aldolase B and FBPase-1 activity at the cellular level through the regulation of their interaction, suggesting that their association confers a catalytic advantage for both enzymes | Rattus norvegicus |
physiological function | fructose-1,6-bisphosphate aldolase B catalyses the reversible reaction of glyceraldehyde-3-phosphate (G3-P) and dihydroxyacetone phosphate (DHAP) to form fructose 1,6-bisphosphate and participates in both gluconeogenesis and glycolysis. In vitro protein-protein interaction analysis between liver fructose 1,6-bisphosphate aldolase B and liver fructose 1,6-bisphosphatase (FBPase-1) shows a specific and regulable interaction between them, whereas aldolase A (muscle isozyme) and FBPase-1 show no interaction, by real-time interaction analysis by surface plasmon resonance. The interaction between aldolase B and FBPase-1 is specific and reversible. The affinity of the aldolase B and FBPase-1 complex is modulated by intermediate metabolites, but only in the presence of K+. A decreased association constant is observed in the presence of adenosine monophosphate, fructose-2,6-bisphosphate, fructose-6-phosphate and inhibitory concentrations of fructose-1,6-bisphosphate. Conversely, the association constant of the complex increases in the presence of dihydroxyacetone phosphate (DHAP) and non-inhibitory concentrations of fructose-1,6-bisphosphate. FBPase-1 guides the cellular localization of aldolase B | Rattus norvegicus |