KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
DL-isocitrate | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 63000, calculation from amino acid sequence | Glycine max |
64000 | - |
2 * 64000, SDS-PAGE | Glycine max |
130000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Glycine max | glyoxylate cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.48 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
-12°C, 20% glycerol, 21 days, 20% loss of activity | Glycine max |
-12°C, 20% glycerol, 7 days, 20% loss of activity | Glycine max |
-12°C, without glycerol, 4 h, 100% loss of activity | Glycine max |
4°C, 20% glycerol, 7 days, 30% loss of activity | Glycine max |
4°C, without glycerol, 7 days, 50% loss of activity | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Glycine max | succinate + glyoxylate | - |
? | |
isocitrate | glyoxylate cycle | Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 64000, SDS-PAGE | Glycine max |
dimer | 2 * 63000, calculation from amino acid sequence | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
heating to temperatures above caused loss of activity | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 30% of optimal activity at pH 7 and pH 9 | Glycine max |