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Literature summary for 4.1.3.17 extracted from

  • Maruyama, K.; Miwa, M.; Tsujii, N.; Nagai, T.; Tomita, N.; Harada, T.; Sobajima, H.; Sugisaki, H.
    Cloning, sequencing, and expression of the gene encoding 4-hydroxy-4-methyl-2-oxoglutarate aldolase from Pseudomonas ochraceae NGJ1 (2001), Biosci. Biotechnol. Biochem., 65, 2701-2709.
    View publication on PubMed
Search for more literature for 4.1.3.17

Cloned(Commentary)

Cloned (Comment) Organism
DNA fragment carrying the gene proA from chromosomal DNA cloned and overproduced in Escherichia coli XL1-Blue MRF' Pseudomonas straminea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 L-4-carboxy-4-hydroxy-2-oxoadipate pH 8.0, 25°C Pseudomonas straminea
0.044
-
 DL-4-carboxy-4-hydroxy-2-oxoadipate pH 8.0, 25°C Pseudomonas straminea
0.15
-
 L-4-carboxy-4-hydroxy-2-oxoadipate pH 8.0, 25°C Pseudomonas straminea
0.24
-
 DL-4-hydroxy-2-oxoglutarate pH 8.0, 25°C Pseudomonas straminea
0.5
-
 oxaloacetate pH 8.0, 25°C Pseudomonas straminea
1.25
-
 DL-4-hydroxy-4-methyl-2-oxoglutarate pH 8.0, 25°C Pseudomonas straminea

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mg2+ Pseudomonas straminea
Mg2+ virtually no activity in absence of Mg2+ or Pi Pseudomonas straminea
Mn2+ can replace Mg2+ Pseudomonas straminea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24070
-
 calculated from amino acid sequence corresponding to gene sequence Pseudomonas straminea
26000
-
 6 * 26000, homohexamer, SDS-PAGE Pseudomonas straminea
160000
-
 gel filtration Pseudomonas straminea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxy-4-methyl-2-oxoglutarate Pseudomonas straminea involved in biosynthesis of gamma-substituted glutamic acid, participates in the alpha-keto acid pathway for degradation of the meta-fission product of protocatechuate, in Pseudomonas ochraceae grown on phthalate the physiolological substrate is not hydroxymethyl glutamic acid but CHA, L-4-carboxy-4-hydroxy-2-oxoadipate, which is cleaved to pyruvate and oxaloacetate pyruvate
-
 r
l-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
-
 pyruvate + oxaloacetate
-
 ?
l-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea NGJ1
-
 pyruvate + oxaloacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas straminea
-
-
-
Pseudomonas straminea NGJ1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant enzyme Pseudomonas straminea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
870
-
-
 Pseudomonas straminea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-4-methyl-2-oxoglutarate involved in biosynthesis of gamma-substituted glutamic acid, participates in the alpha-keto acid pathway for degradation of the meta-fission product of protocatechuate, in Pseudomonas ochraceae grown on phthalate the physiolological substrate is not hydroxymethyl glutamic acid but CHA, L-4-carboxy-4-hydroxy-2-oxoadipate, which is cleaved to pyruvate and oxaloacetate Pseudomonas straminea pyruvate
-
 r
D-4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas straminea pyruvate + oxaloacetate
-
 ?
D-4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas straminea NGJ1 pyruvate + oxaloacetate
-
 ?
DL-4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas straminea pyruvate + oxaloacetate
-
 ?
DL-4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas straminea NGJ1 pyruvate + oxaloacetate
-
 ?
DL-4-hydroxy-2-oxoglutarate
-
 Pseudomonas straminea pyruvate + glyoxylate
-
 ?
DL-4-hydroxy-4-methyl-2-oxoglutarate
-
 Pseudomonas straminea pyruvate
-
 ?
DL-4-hydroxy-4-methyl-2-oxoglutarate
-
 Pseudomonas straminea NGJ1 pyruvate
-
 ?
l-4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas straminea pyruvate + oxaloacetate
-
 ?
l-4-carboxy-4-hydroxy-2-oxoadipate
-
 Pseudomonas straminea NGJ1 pyruvate + oxaloacetate
-
 ?
oxaloacetate decarboxylation Pseudomonas straminea ?
-
 ?
oxaloacetate decarboxylation Pseudomonas straminea NGJ1 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 26000, homohexamer, SDS-PAGE Pseudomonas straminea

Synonyms

Synonyms Comment Organism
4-hydroxy-4-methyl-2-oxoglutarate aldolase
-
 Pseudomonas straminea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas straminea

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas straminea isoelectric focusing
-
 5
Pseudomonas straminea predicted pI
-
 5.7