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Literature summary for 4.1.99.2 extracted from

  • Nagasawa, T.; Utagawa, T.; Goto, J.; Kim, C.J.; Tani Y.; Kumagai, H.; Yamada, H.
    Syntheses of L-tyrosine-related amino acids by tyrosine phenol-lyase of Citrobacter intermedius (1981), Eur. J. Biochem., 117, 33-40.
    View publication on PubMed
Search for more literature for 4.1.99.2

Inhibitors

Inhibitors Comment Organism Structure
1,2-dihydroxybenzene cleavage of Tyr Citrobacter intermedius
1,3-dihydroxybenzene cleavage of Tyr Citrobacter intermedius
2-Aminophenol cleavage of Tyr Citrobacter intermedius
2-bromophenol cleavage of Tyr Citrobacter intermedius
2-chlorophenol cleavage of Tyr Citrobacter intermedius
2-Fluorophenol cleavage of Tyr Citrobacter intermedius
2-iodophenol cleavage of Tyr Citrobacter intermedius
2-methylphenol cleavage of Tyr Citrobacter intermedius
3-Aminophenol cleavage of Tyr Citrobacter intermedius
3-bromophenol cleavage of Tyr Citrobacter intermedius
3-chlorophenol cleavage of Tyr Citrobacter intermedius
3-Fluorophenol cleavage of Tyr Citrobacter intermedius
3-iodophenol cleavage of Tyr Citrobacter intermedius
3-methylphenol cleavage of Tyr Citrobacter intermedius
4-Aminophenol cleavage of Tyr Citrobacter intermedius
4-hydroxypyridine cleavage of Tyr Citrobacter intermedius
phenol cleavage of Tyr Citrobacter intermedius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 p-L-Tyr
-
 Citrobacter intermedius
0.35
-
 2-fluoro-L-Tyr
-
 Citrobacter intermedius
0.4
-
 3-fluoro-L-Tyr
-
 Citrobacter intermedius
0.7
-
 2-Chloro-L-Tyr
-
 Citrobacter intermedius
1.4
-
 3-bromo-L-Tyr
-
 Citrobacter intermedius
1.6
-
 2-methoxy-L-Tyr
-
 Citrobacter intermedius
2
-
 L-Phe
-
 Citrobacter intermedius
3
-
 3-Chloro-L-Tyr
-
 Citrobacter intermedius
3.5
-
 2-bromo-L-Tyr
-
 Citrobacter intermedius
3.7
-
 2-Methyl-L-Tyr
-
 Citrobacter intermedius
4.1
-
 3-methyl-L-Tyr
-
 Citrobacter intermedius

Organism

Organism UniProt Comment Textmining
Citrobacter intermedius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Citrobacter intermedius

Reaction

Reaction Comment Organism Reaction ID
L-tyrosine + H2O = phenol + pyruvate + NH3 NH3 is the first substrate which interacts with bound pyridoxal 5'-phosphate, pyruvate is the second substrate Citrobacter intermedius
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.65
-
-
 Citrobacter intermedius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-bromo-L-Tyr + H2O
-
 Citrobacter intermedius 2-bromophenol + pyruvate + NH3
-
 r
2-bromophenol + pyruvate + NH3
-
 Citrobacter intermedius 2-bromo-L-Tyr + H2O
-
 r
2-chloro-L-Tyr + H2O
-
 Citrobacter intermedius 2-chlorophenol + pyruvate + NH3
-
 r
2-chlorophenol + pyruvate + NH3
-
 Citrobacter intermedius 2-chloro-L-Tyr + H2O
-
 r
2-fluoro-L-Tyr + H2O
-
 Citrobacter intermedius 2-fluorophenol + pyruvate + NH3
-
 r
2-fluorophenol + pyruvate + NH3
-
 Citrobacter intermedius 2-fluoro-L-Tyr + H2O
-
 r
2-methoxy-L-Tyr + H2O
-
 Citrobacter intermedius 2-methoxyphenol + pyruvate + NH3
-
 r
2-methoxyphenol + pyruvate + NH3
-
 Citrobacter intermedius 2-methoxy-L-Tyr + H2O
-
 r
2-methyl-L-Tyr + H2O
-
 Citrobacter intermedius 2-methylphenol + pyruvate + NH3
-
 r
2-methylphenol + pyruvate + NH3
-
 Citrobacter intermedius 2-methyl-L-Tyr + H2O
-
 r
3-bromo-L-Tyr + H2O
-
 Citrobacter intermedius 3-bromophenol + pyruvate + NH3
-
 r
3-bromophenol + pyruvate + NH3
-
 Citrobacter intermedius 3-bromo-L-Tyr + H2O
-
 r
3-chloro-L-Tyr + H2O
-
 Citrobacter intermedius 3-chlorophenol + pyruvate + NH3
-
 r
3-chlorophenol + pyruvate + NH3
-
 Citrobacter intermedius 3-chloro-L-Tyr + H2O
-
 r
3-fluoro-L-Tyr + H2O
-
 Citrobacter intermedius 3-fluorophenol + pyruvate + NH3
-
 r
3-fluorophenol + pyruvate + NH3
-
 Citrobacter intermedius 3-fluoro-L-Tyr + H2O
-
 r
3-methyl-L-Tyr + H2O
-
 Citrobacter intermedius 3-methylphenol + pyruvate + NH3
-
 r
3-methylphenol + pyruvate + NH3
-
 Citrobacter intermedius 3-methyl-L-Tyr + H2O
-
 r
L-Phe + H2O
-
 Citrobacter intermedius ?
-
 r
phenol + pyruvate + NH3
-
 Citrobacter intermedius Tyr + H2O
-
 r
Tyr + H2O L-Tyr Citrobacter intermedius phenol + pyruvate + NH3
-
 r

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate cofactor Citrobacter intermedius