Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
in complex with Ca2+, sitting drop vapour diffusion method, with 22.5% PEG 4000 in MES-NaOH pH 5.8 and 0.1 M lithium chloride as the precipitating agent | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the native enzyme contains four Ca2+ ions | Pyrococcus horikoshii | |
Zn2+ | zinc-containing metalloenzyme, the asymmetric unit contains a catalytic Zn2+ ion | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59257 | strain OT3 | - |
Pyrococcus horikoshii OT-3 | O59257 | strain OT3 | - |
Purification (Comment) | Organism |
---|---|
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT-20-I column chromatography, and Superdex 200pg gel filtration | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Pyrococcus horikoshii | H2CO3 | - |
r | |
CO2 + H2O | - |
Pyrococcus horikoshii OT-3 | H2CO3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
carbonic anhydrase | - |
Pyrococcus horikoshii |