Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | 62% residual activity at 1 mM | Bacillus subtilis | |
Ca2+ | 72% residual activity at 1 mM | Bacillus subtilis | |
Cl- | 10% residual activity at 1 mM | Bacillus subtilis | |
EDTA | complete inhibition at 1 mM | Bacillus subtilis | |
HCO3- | 7% residual activity at 1 mM | Bacillus subtilis | |
Hg2+ | complete inhibition at 1 mM | Bacillus subtilis | |
Mn2+ | 73% residual activity at 1 mM | Bacillus subtilis | |
NO2- | 41% residual activity at 1 mM | Bacillus subtilis | |
NO3- | 36% residual activity at 1 mM | Bacillus subtilis | |
Pb2+ | 33% residual activity at 1 mM | Bacillus subtilis | |
sulfanilamide | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.09 | - |
4-nitrophenyl acetate | in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 305% activity at 1 mM | Bacillus subtilis | |
Cu2+ | 165% activity at 1 mM | Bacillus subtilis | |
Fe3+ | 281% activity at 1 mM | Bacillus subtilis | |
Mg2+ | 107% activity at 1 mM | Bacillus subtilis | |
SO42- | 126% activity at 1 mM | Bacillus subtilis | |
Zn2+ | 473% activity at 1 mM | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis SA3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-75 gel filtration, DEAE cellulose column chromatography, and Sepharose-4B-L tyrosine sulfanilamide column chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.62 | - |
crude extract, using 4-nitrophenyl acetate as substrate, at pH 8.3 and 37°C | Bacillus subtilis |
737.8 | - |
after 456.6fold purification, using 4-nitrophenyl acetate as substrate, at pH 8.3 and 37°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Bacillus subtilis SA3 | 4-nitrophenol + acetate | - |
? | |
CO2 + H2O | - |
Bacillus subtilis | H2CO3 | - |
r | |
CO2 + H2O | - |
Bacillus subtilis SA3 | H2CO3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
carbonate hydro-lyase | - |
Bacillus subtilis |
carbonic anhydrase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme is not stable at temperatures of 60°C and above | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 13 | activity is completely lost at a moderately acidic pH of 4.0 and a highly basic pH of 13.0 | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.099 | - |
sulfanilamide | in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C | Bacillus subtilis | |
0.22 | - |
acetazolamide | in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | isoelectric focusing | - |
6.1 |