Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain JM109 | Klebsiella oxytoca |
General Stability | Organism |
---|---|
calcium-deprived apoenzyme is unstable when incubated at 37°C in the absence of both Ca2+ and substrate | Klebsiella oxytoca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | EDTA inhibition of apoenzyme is reversible at least in the initial phase | Klebsiella oxytoca | |
EGTA | - |
Klebsiella oxytoca | |
additional information | no inhibition by 5 mM of 1,10-phenanthroline, 2,2'-dipyridyl, citrate, succinate, tartrate, malate, and salicylate | Klebsiella oxytoca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.6-1.9 mol/mol of enzyme. CaCl2 and substrate show not only a stabilizing effect but also some activating effect of about 30% | Klebsiella oxytoca | |
Cr2+ | 0.10 mol/mol of enzyme | Klebsiella oxytoca | |
Cu2+ | 0.16 mol/mol of enzyme | Klebsiella oxytoca | |
Fe2+ | 0.09 mol/mol of enzyme | Klebsiella oxytoca | |
K+ | selectively activates diol dehydratase | Klebsiella oxytoca | |
Mg2+ | 0.19-0.26 mol/mol of enzyme | Klebsiella oxytoca | |
Mn2+ | 0.09 mol/mol of enzyme | Klebsiella oxytoca | |
additional information | no requirements of divalentmetal ions for catalysis, metal contents in purified recombinant enzyme | Klebsiella oxytoca | |
NH4+ | selectively activates diol dehydratase | Klebsiella oxytoca | |
Zn2+ | 0.12 mol/mol of enzyme | Klebsiella oxytoca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
207300 | - |
- |
Klebsiella oxytoca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propane-1,2-diol | Klebsiella oxytoca | - |
propanal + H2O | - |
? | |
propane-1,2-diol | Klebsiella oxytoca ATCC 8724 | - |
propanal + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | - |
- |
- |
Klebsiella oxytoca ATCC 8724 | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
reconstitution of calcium-containing apoenzyme from calcium-deprived apoenzyme and Ca2+, overview | Klebsiella oxytoca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ATCC8724 cells grown without aeration in glycerol-1,2-propanediol medium | Klebsiella oxytoca | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propane-1,2-diol | - |
Klebsiella oxytoca | propanal + H2O | - |
? | |
propane-1,2-diol | - |
Klebsiella oxytoca ATCC 8724 | propanal + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosylcobalamin-dependent diol dehydratase | - |
Klebsiella oxytoca |
AdoCbl-dependent diol dehydratase | - |
Klebsiella oxytoca |
coenzyme B12-dependent diol dehydratase | - |
Klebsiella oxytoca |
diol dehydratase | - |
Klebsiella oxytoca |
DL-1,2-propanediol hydrolyase | - |
Klebsiella oxytoca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Klebsiella oxytoca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Klebsiella oxytoca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | i.e. coenzyme B12 or AdoCbl, dependent on | Klebsiella oxytoca |