Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Lentilactobacillus diolivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Lentilactobacillus diolivorans | |
glycerol | suicide inactivation, Km value of 5.8 mM | Lentilactobacillus diolivorans | |
Mg2+ | slight inhibition | Lentilactobacillus diolivorans | |
Mn2+ | - |
Lentilactobacillus diolivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Propane-1,2-diol | at pH 8.6 and 37°C | Lentilactobacillus diolivorans | |
10.5 | - |
ethane-1,2-diol | at pH 8.6 and 37°C | Lentilactobacillus diolivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | enzyme activity increases by 1 mM K+ | Lentilactobacillus diolivorans | |
additional information | not affected by Na+ and Li+ | Lentilactobacillus diolivorans | |
NH4+ | enzyme activity increases by 1 mM NH4+ | Lentilactobacillus diolivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19800 | - |
2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE | Lentilactobacillus diolivorans |
35000 | - |
2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE | Lentilactobacillus diolivorans |
61100 | - |
2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE | Lentilactobacillus diolivorans |
210000 | - |
gel filtration | Lentilactobacillus diolivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propane-1,2-diol | Lentilactobacillus diolivorans | - |
propanal + H2O | - |
? | |
propane-1,2-diol | Lentilactobacillus diolivorans DSM 14421 | - |
propanal + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentilactobacillus diolivorans | - |
- |
- |
Lentilactobacillus diolivorans DSM 14421 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethane-1,2-diol | - |
Lentilactobacillus diolivorans | ethanal + H2O | - |
? | |
ethane-1,2-diol | - |
Lentilactobacillus diolivorans DSM 14421 | ethanal + H2O | - |
? | |
propane-1,2-diol | - |
Lentilactobacillus diolivorans | propanal + H2O | - |
? | |
propane-1,2-diol | - |
Lentilactobacillus diolivorans DSM 14421 | propanal + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | 2 * 61100 + 2 * 35000 + 2 * 19800, SDS-PAGE | Lentilactobacillus diolivorans |
Synonyms | Comment | Organism |
---|---|---|
coenzyme-B12-dependent diol dehydratase | - |
Lentilactobacillus diolivorans |
DDH | - |
Lentilactobacillus diolivorans |
GldCDE | - |
Lentilactobacillus diolivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Lentilactobacillus diolivorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | 50 | the enzyme retains more than 70% of its activity between 30 and 40°C but less than 20% of its activity at 16 and 50°C | Lentilactobacillus diolivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Lentilactobacillus diolivorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | less than 10% activity is observed at pH 4.5 and pH 10.5 | Lentilactobacillus diolivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | dependent on, Km value of 0.0015 mM | Lentilactobacillus diolivorans |