Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and diverse pount and deletion mutants | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C381A | site-directed mutagenesis | Saccharomyces cerevisiae |
C447A | site-directed mutagenesis | Saccharomyces cerevisiae |
H170A | site-directed mutagenesis | Saccharomyces cerevisiae |
additional information | construction of diverse deletion mutants of aconitase, overview. Deletion of six C-terminal amino acids does not eliminate enzymatic activity but abolishes dual targeting | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | aconitase in yeast is a single translation product, which is dual targeted and distributed between the mitochondria and the cytosol by a unique mechanism involving reverse translocation, dual localization, detailed overview | Saccharomyces cerevisiae | 5829 | - |
mitochondrion | aconitase in yeast is a single translation product, which is dual targeted and distributed between the mitochondria and the cytosol by a unique mechanism involving reverse translocation, dual localization, detailed overview | Saccharomyces cerevisiae | 5739 | - |
additional information | subcellular localization study, overview. All mutant aconitase protein molecules are partially imported into mitochondria, then the N-terminal mitochondrial targeting sequence is cleaved off by the mitochondrial processing peptidase. After the cleavage, a subpopulation of the protein molecules moves back into the cytosol by reverse translocation. The aconitase C-terminal domain confers dual targeting | Saccharomyces cerevisiae | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
Aco1 | - |
Saccharomyces cerevisiae |
aconitase | - |
Saccharomyces cerevisiae |