Any feedback?
Literature summary for 4.2.1.53 extracted from
- Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis (2012), Appl. Microbiol. Biotechnol., 95, 929-937.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 120% activity at 4% (v/v) ethanol | Lysinibacillus fusiformis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Lysinibacillus fusiformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | less than 60% residual activity at 6% (v/v) ethanol | Lysinibacillus fusiformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
oleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 0.58 | - |
linoleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 0.76 | - |
palmitoleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 0.77 | - |
gamma-linolenate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
2 * 69000, SDS-PAGE | Lysinibacillus fusiformis |
| 69200 | - |
2 * 69200, calculated from amino acid sequence | Lysinibacillus fusiformis |
| 138000 | - |
gel filtration | Lysinibacillus fusiformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleate + H2O | Lysinibacillus fusiformis | highest activity | (R)-10-hydroxystearate | - |
? | |
| oleate + H2O | Lysinibacillus fusiformis KCTC 3454 | highest activity | (R)-10-hydroxystearate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus fusiformis | - |
- |
- |
| Lysinibacillus fusiformis KCTC 3454 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap column chromatography | Lysinibacillus fusiformis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
with alpha-linolenate as substrate, at pH 6.5 and 35°C | Lysinibacillus fusiformis |
| 30 | - |
with myristoleate as substrate, at pH 6.5 and 35°C | Lysinibacillus fusiformis |
| 110 | - |
with linoleate as substrate, at pH 6.5 and 35°C | Lysinibacillus fusiformis |
| 160 | - |
with gamma-linolenate as substrate, at pH 6.5 and 35°C | Lysinibacillus fusiformis |
| 550 | - |
with palmitoleate as substrate, at pH 6.5 and 35°C | Lysinibacillus fusiformis |
| 1580 | - |
with oleate as substrate, at pH 6.5 and 35°C | Lysinibacillus fusiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenate + H2O | - |
Lysinibacillus fusiformis | (R)-10-hydroxy-12,15(Z,Z)-octadecadienoate | - |
? | |
| alpha-linolenate + H2O | - |
Lysinibacillus fusiformis KCTC 3454 | (R)-10-hydroxy-12,15(Z,Z)-octadecadienoate | - |
? | |
| gamma-linolenate + H2O | - |
Lysinibacillus fusiformis | (R)-10-hydroxy-6,12-(Z,Z)-octadecadienoate | - |
? | |
| gamma-linolenate + H2O | - |
Lysinibacillus fusiformis KCTC 3454 | (R)-10-hydroxy-6,12-(Z,Z)-octadecadienoate | - |
? | |
| linoleate + H2O | - |
Lysinibacillus fusiformis | (R)-10-hydroxy-12(Z)-octadecenoate | - |
? | |
| linoleate + H2O | - |
Lysinibacillus fusiformis KCTC 3454 | (R)-10-hydroxy-12(Z)-octadecenoate | - |
? | |
| myristoleate + H2O | - |
Lysinibacillus fusiformis | (R)-10-hydroxymyristate | - |
? | |
| myristoleate + H2O | - |
Lysinibacillus fusiformis KCTC 3454 | (R)-10-hydroxymyristate | - |
? | |
| oleate + H2O | highest activity | Lysinibacillus fusiformis | (R)-10-hydroxystearate | - |
? | |
| oleate + H2O | highest activity | Lysinibacillus fusiformis KCTC 3454 | (R)-10-hydroxystearate | - |
? | |
| palmitoleate + H2O | - |
Lysinibacillus fusiformis | (R)-10-hydroxypalmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 69000, SDS-PAGE | Lysinibacillus fusiformis |
| homodimer | 2 * 69200, calculated from amino acid sequence | Lysinibacillus fusiformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Lysinibacillus fusiformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | more than 60% activity between 20 and 45°C | Lysinibacillus fusiformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.33 | - |
linoleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 2.17 | - |
gamma-linolenate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 7.17 | - |
palmitoleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 14.17 | - |
oleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Lysinibacillus fusiformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | more than 50% activity between pH 5.5 and 7.5 | Lysinibacillus fusiformis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.33 | - |
linoleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 2.67 | - |
gamma-linolenate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 9.33 | - |
palmitoleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
| 26.17 | - |
oleate | at pH 6.5 and 35°C | Lysinibacillus fusiformis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
