Literature summary for 4.2.1.53 extracted from

Hiseni, A.; Arends, I.; Otten, L.
New cofactor-independent hydration biocatalysts Structural, biochemical, and biocatalytic characteristics of carotenoid and oleate hydratases (2015), ChemCatChem, 7, 29-37 .

No PubMed abstract available

Application

Application	Comment	Organism
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Lactobacillus acidophilus
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Stenotrophomonas maltophilia
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Elizabethkingia meningoseptica
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Macrococcus caseolyticus
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Streptococcus pyogenes
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Lysinibacillus fusiformis
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Lacticaseibacillus rhamnosus
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Lactiplantibacillus plantarum
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Bifidobacterium breve
synthesis	(R)-10-hydroxyoctadecanoic acid, produced by the oleate hydratase is a precursor for gamma-dodecalactone which is an important fragrance and flavour compound	Bifidobacterium animalis subsp. lactis
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Lactobacillus acidophilus
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Stenotrophomonas maltophilia
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Elizabethkingia meningoseptica
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Macrococcus caseolyticus
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Streptococcus pyogenes
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Lysinibacillus fusiformis
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Lacticaseibacillus rhamnosus
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Lactiplantibacillus plantarum
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Bifidobacterium breve
synthesis	one of the largest bottlenecks in producing lactones is the hydroxylation of the fatty acids. The exploitation of oleate hydratase to hydrate oleic acid, which can easily be turned into gamma-dodecalactone after beta-elimination, is therefore the perfect solution to produce this valuable compound from cheap renewable materials (e.g. vegetable oil). The specificity and enantioselectivity of the enzyme will provide the correct lactone	Bifidobacterium animalis subsp. lactis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Lactobacillus acidophilus
expression in Escherichia coli	Stenotrophomonas maltophilia
expression in Escherichia coli	Elizabethkingia meningoseptica
expression in Escherichia coli	Macrococcus caseolyticus
expression in Escherichia coli	Streptococcus pyogenes
expression in Escherichia coli	Lysinibacillus fusiformis
expression in Escherichia coli	Lacticaseibacillus rhamnosus
expression in Escherichia coli	Lactiplantibacillus plantarum
expression in Escherichia coli	Bifidobacterium breve
expression in Escherichia coli	Bifidobacterium animalis subsp. lactis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
67000	-	-	Lactobacillus acidophilus
67000	-	-	Stenotrophomonas maltophilia
67000	-	-	Elizabethkingia meningoseptica
67000	-	-	Macrococcus caseolyticus
67000	-	-	Streptococcus pyogenes
67000	-	-	Lysinibacillus fusiformis
67000	-	-	Lacticaseibacillus rhamnosus
67000	-	-	Lactiplantibacillus plantarum
67000	-	-	Bifidobacterium breve
82000	-	-	Bifidobacterium animalis subsp. lactis

Organism

Organism	UniProt	Comment	Textmining
Bifidobacterium animalis subsp. lactis	A0A1C7FUY1	-	-
Bifidobacterium breve	A0A2N6TXX1	-	-
Elizabethkingia meningoseptica	C7DLJ6	-	-
Lacticaseibacillus rhamnosus	A0A249DBU8	-	-
Lactiplantibacillus plantarum	A0A0G9FDC3	-	-
Lactobacillus acidophilus	-	-	-
Lysinibacillus fusiformis	A0A1E4R6K3	-	-
Macrococcus caseolyticus	B9E972	-	-
Stenotrophomonas maltophilia	-	-	-
Streptococcus pyogenes	B5XK69	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(6Z,9Z,12Z)-octadec-6,9,12-trienoic acid + H2O	-	Stenotrophomonas maltophilia	(6Z,12Z)-10-hydroxyoctadec-6,12-dienoic acid	-	?
(6Z,9Z,12Z)-octadec-6,9,12-trienoic acid + H2O	-	Macrococcus caseolyticus	(6Z,12Z)-10-hydroxyoctadec-6,12-dienoic acid	-	?
(6Z,9Z,12Z)-octadec-6,9,12-trienoic acid + H2O	-	Streptococcus pyogenes	(6Z,12Z)-10-hydroxyoctadec-6,12-dienoic acid	-	?
(6Z,9Z,12Z)-octadec-6,9,12-trienoic acid + H2O	-	Lysinibacillus fusiformis	(6Z,12Z)-10-hydroxyoctadec-6,12-dienoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Lactobacillus acidophilus	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Stenotrophomonas maltophilia	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Elizabethkingia meningoseptica	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Macrococcus caseolyticus	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Streptococcus pyogenes	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Lysinibacillus fusiformis	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Lacticaseibacillus rhamnosus	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Lactiplantibacillus plantarum	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Bifidobacterium breve	10-hydroxyhexadecanoic acid	-	?
(9Z)-hexadec-9-enoic acid + H2O	-	Bifidobacterium animalis subsp. lactis	10-hydroxyhexadecanoic acid	-	?
(9Z)-octadec-9-enoic acid + H2O	-	Stenotrophomonas maltophilia	(R)-10-hydroxyoctadecanoic acid	-	?
(9Z)-octadec-9-enoic acid + H2O	-	Macrococcus caseolyticus	(R)-10-hydroxyoctadecanoic acid	-	?
(9Z)-octadec-9-enoic acid + H2O	-	Streptococcus pyogenes	(R)-10-hydroxyoctadecanoic acid	-	?
(9Z)-octadec-9-enoic acid + H2O	-	Lysinibacillus fusiformis	(R)-10-hydroxyoctadecanoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Lactobacillus acidophilus	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Stenotrophomonas maltophilia	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Elizabethkingia meningoseptica	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Macrococcus caseolyticus	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Streptococcus pyogenes	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Lysinibacillus fusiformis	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Lacticaseibacillus rhamnosus	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Lactiplantibacillus plantarum	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Bifidobacterium breve	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z)-octadec-9,12-dienoic acid + H2O	-	Bifidobacterium animalis subsp. lactis	(12Z)-10-hydroxyoctadec-12-enoic acid	-	?
(9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O	-	Stenotrophomonas maltophilia	(12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid	-	?
(9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O	-	Elizabethkingia meningoseptica	(12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid	-	?
(9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O	-	Macrococcus caseolyticus	(12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid	-	?
(9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O	-	Streptococcus pyogenes	(12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid	-	?
(9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O	-	Lysinibacillus fusiformis	(12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Stenotrophomonas maltophilia
dimer	-	Macrococcus caseolyticus
dimer	-	Streptococcus pyogenes
dimer	-	Lysinibacillus fusiformis

Synonyms

Synonyms	Comment	Organism
OHase	-	Lactobacillus acidophilus
OHase	-	Stenotrophomonas maltophilia
OHase	-	Elizabethkingia meningoseptica
OHase	-	Macrococcus caseolyticus
OHase	-	Streptococcus pyogenes
OHase	-	Lysinibacillus fusiformis
OHase	-	Lacticaseibacillus rhamnosus
OHase	-	Lactiplantibacillus plantarum
OHase	-	Bifidobacterium breve
OHase	-	Bifidobacterium animalis subsp. lactis

Cofactor

Cofactor	Comment	Organism
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Lactobacillus acidophilus
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Stenotrophomonas maltophilia
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Elizabethkingia meningoseptica
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Macrococcus caseolyticus
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Streptococcus pyogenes
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Lysinibacillus fusiformis
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Lacticaseibacillus rhamnosus
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Lactiplantibacillus plantarum
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Bifidobacterium breve
FAD	with the multiple sequence alignment a motif indicative of FAD binding is identified	Bifidobacterium animalis subsp. lactis