Cloned (Comment) | Organism |
---|---|
overexpression of mutant Y408F in Escherichia coli | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
5 mg/ml Y408 mutant enzyme complexed with tetra- and hexasaccharide hyaluronan substrates, in 10 mM Tris-HCl, pH 7.4, 2 mM EDTA, 1 mM DL-dithiothreitol, against 100 mM sodium cacodylate, pH 6.0, 2.9 M ammonium sulfate, 5 mM EDTA, hanging drop vapour diffusion method, 0.002 ml equal volumes of protein and reservoir solution, 22°C, equilibration against 1 ml reservoir solution, several days, X-ray diffraction structure determination and analysis at 1.52-2.0 A resolution | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
Y408F | enzyme substrate complex structure determination by crystallization | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan | Streptococcus pneumoniae | extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | Asn349, His399, and Tyr408 are involved in catalysis, flexible, allosteric behavior of the enzyme, mechanism, active site structure and substrate binding and interactions, overview | Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in different isolated strains from several sources, overview | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan | extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue | Streptococcus pneumoniae | ? | - |
? | |
hyaluronan | - |
Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide units | ? | |
hyaluronan hexasaccharide | the longest segment that binds entirely within the active site, a cleft between alpha- and beta-domain | Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide units | ? | |
hyaluronan tetrasaccharide | - |
Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide units | ? |
Synonyms | Comment | Organism |
---|---|---|
spnHL | - |
Streptococcus pneumoniae |