Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value is 0.13 mg/ml for 22% esterified pectin, the KM-value is 0.15 mg/ml for polygalacturonic acid | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolute requirement | Bacillus subtilis | |
Hg2+ | 1 mM, can replace Ca2+ in activation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin | of methyl esterification degree from 22% to 89%, maximal activity on 22% esterified citrus pectin | Bacillus subtilis | ? | - |
? | |
polygalacturonate | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pectate lyase C | - |
Bacillus subtilis |
pelC | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 60% of maximal activity, 80°C: about 60% of maximal activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | pH 9: about 40% of maximal activity, pH 11: about 50% of maximal activity | Bacillus subtilis |