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Literature summary for 4.2.2.2 extracted from
- Biochemical characteristics of an alkaline pectate lyase PelA from Volvariella volvacea roles of the highly conserved N-glycosylation site in its secretion and activity (2015), Appl. Microbiol. Biotechnol., 99, 3447-3458 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N198A | 95% of wild-type activity | Volvariella volvacea |
| N198D | 77% of wild-type activity | Volvariella volvacea |
| N198Q | 72% of wild-type activity | Volvariella volvacea |
| N95A | 38% of wild-type activity | Volvariella volvacea |
| N95D | 51% of wild-type activity | Volvariella volvacea |
| N95Q | 54% of wild-type activity | Volvariella volvacea |
| N95S | 81% of wild-type activity | Volvariella volvacea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 2% of initial activity | Volvariella volvacea |  |
| SDS | 1 mM, 57% of initial activity | Volvariella volvacea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.1 mM, 180% of initial activity. Presence of Ca2+ enhances thermal stability | Volvariella volvacea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Volvariella volvacea | A0A097PUC5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sequence contains one conserved and one nonconserved potential N-glycosylation site (N-X-S/T) at the residues N95 and N198, respectively. Protein has only one N-glycan attached to residue N95 | Volvariella volvacea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.07 | - |
pH 10, 60°C | Volvariella volvacea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | - |
Volvariella volvacea | unsaturated oligogalacturonides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34088, calculated from sequence, x * 37500 and x * 35000, SDS-PAGE, glycosylated and unglycosylated protein, respectively | Volvariella volvacea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PelA | - |
Volvariella volvacea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
mutant N198Q | Volvariella volvacea |
| 60 | - |
wild-type and mutants | Volvariella volvacea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1h, more than 60% residual activity | Volvariella volvacea |
| 47.9 | - |
melting temperature, mutant N198Q | Volvariella volvacea |
| 48.7 | - |
melting temperature, mutant N198D | Volvariella volvacea |
| 48.9 | - |
melting temperature, mutant N198A | Volvariella volvacea |
| 50.7 | - |
melting temperature, mutant N95S | Volvariella volvacea |
| 52 | - |
melting temperature, wild-type | Volvariella volvacea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Volvariella volvacea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | 4°C, 24 h, more than 80% residual activity | Volvariella volvacea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Volvariella volvacea | calculated from sequence | - |
8.8 |
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