Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and transient Agrobacterium-mediated expression in Nicotiana benthamiana | Pseudolarix amabilis |
Crystallization (Comment) | Organism |
---|---|
homology modeling and docking of substrate. The Mg2+-coordinating DDxxD motif and arginine residues R558, R560, and R736 critical for catalysis in Abies grandis are conserved in TPS8 | Pseudolarix amabilis |
Protein Variants | Comment | Organism |
---|---|---|
R558A | complete loss of activity | Pseudolarix amabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Pseudolarix amabilis | - |
pseudolaratriene + diphosphate | - |
? | |
additional information | Pseudolarix amabilis | no product is formed with geranyl diphosphate or farnesyl diphosphate or in assays with class II diterpene synthases forming copalyl diphosphate of ent- or (+)-stereochemistry | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudolarix amabilis | A0A1L6Z3A0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bark | expression predominantly in roots and in bark | Pseudolarix amabilis | - |
needle | expression near the limit of detection in needle tissue | Pseudolarix amabilis | - |
root | expression predominantly in roots and in bark | Pseudolarix amabilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Pseudolarix amabilis | pseudolaratriene + diphosphate | - |
? | |
additional information | no product is formed with geranyl diphosphate or farnesyl diphosphate or in assays with class II diterpene synthases forming copalyl diphosphate of ent- or (+)-stereochemistry | Pseudolarix amabilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pseudolarene synthase | - |
Pseudolarix amabilis |
TPS8 | - |
Pseudolarix amabilis |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme catalyzes the first committed step in pseudolaric acid B biosynthesis, showing a distinct carbocation-driven reaction mechanism en route to the 5,7-trans-fused bicyclic pseudolaratriene scaffold | Pseudolarix amabilis |