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Literature summary for 4.2.3.49 extracted from

  • Su, P.; Hu, T.; Liu, Y.; Tong, Y.; Guan, H.; Zhang, Y.; Zhou, J.; Huang, L.; Gao, W.
    Functional characterization of NES and GES responsible for the biosynthesis of (E)-nerolidol and (E,E)-geranyllinalool in Tripterygium wilfordii (2017), Sci. Rep., 7, 40851 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Transetta (DE3) cells Tripterygium wilfordii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0127
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C Tripterygium wilfordii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highest activity at 10 mM Mg2+ Tripterygium wilfordii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O Tripterygium wilfordii
-
 (3R,6E)-nerolidol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tripterygium wilfordii A0A1S5TFB0
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Tripterygium wilfordii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
 Tripterygium wilfordii (3R,6E)-nerolidol + diphosphate
-
 ?
geranylgeranyl diphosphate
-
 Tripterygium wilfordii (E,E)-geranyllinalool + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 63020, calculated from amino acid sequence Tripterygium wilfordii

Synonyms

Synonyms Comment Organism
NES
-
 Tripterygium wilfordii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.371
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C Tripterygium wilfordii

pI Value

Organism Comment pI Value Maximum pI Value
Tripterygium wilfordii calculated from amino acid sequence
-
 5.3

Expression

Organism Comment Expression
Tripterygium wilfordii enzyme expression increases by several fold in the suspension cells treated with alamethicin up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C Tripterygium wilfordii