Application | Comment | Organism |
---|---|---|
drug development | inhibition of AP endonuclease may be effective in decreasing the dose requirements of chemotherapeutics used in the treatment of cancer as well as other diseases. | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM included in assay medium, required divalent cation | Homo sapiens | |
Mn2+ | Glu in amino acid position 96 binds to the divalent cation | Homo sapiens | |
Na+ | 65 mM included in assay medium | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing apurinic/apyrimidinic site | Homo sapiens | - |
DNA fragments | - |
? | |
additional information | Homo sapiens | a DNA base excision repair enzyme with a wide variety of functions, including AP endonuclease (cleaving an AP site 5' to a deoxyribose phosphate moiety), 3' exonuclease, 3' phosphodiesterase, 3' phosphatase, RNaseH, and 5' endonuclease activities | ? | - |
? | |
additional information | Homo sapiens | AP endo acts by a one-step associative phosphoryl transfer mechanism on a THF-containing substrate | ? | - |
? | |
additional information | Homo sapiens | AP endo acts on many types of DNA substrate molecules but demonstrates the most robust activity when acting as a class II AP endonuclease | ? | - |
? | |
additional information | Homo sapiens | major factor in the maintenance of the integrity of the human genome | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
750 nM substrate and 100 nM enzyme and a reaction time of 3 h is sufficient to convert most, if not all, of the Rp stereoisomer substrate DNA into product | Homo sapiens |
additional information | - |
the enzyme is responsible for eliminating as many as 105 potentially mutagenic and genotoxic lesions from the genome of each cell every day | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing apurinic/apyrimidinic site | - |
Homo sapiens | DNA fragments | - |
? | |
DNA containing apurinic/apyrimidinic sites | - |
Homo sapiens | ? | - |
? | |
additional information | a DNA base excision repair enzyme with a wide variety of functions, including AP endonuclease (cleaving an AP site 5' to a deoxyribose phosphate moiety), 3' exonuclease, 3' phosphodiesterase, 3' phosphatase, RNaseH, and 5' endonuclease activities | Homo sapiens | ? | - |
? | |
additional information | AP endo acts by a one-step associative phosphoryl transfer mechanism on a THF-containing substrate | Homo sapiens | ? | - |
? | |
additional information | AP endo acts on many types of DNA substrate molecules but demonstrates the most robust activity when acting as a class II AP endonuclease | Homo sapiens | ? | - |
? | |
additional information | major factor in the maintenance of the integrity of the human genome | Homo sapiens | ? | - |
? | |
additional information | AP endo cleaves the Rp but not the Sp stereoisomer of DNA phosphorothioate oligomers, albeit slowly with respect to a phosphodiester substrate | Homo sapiens | ? | - |
? | |
additional information | The ratio of AP endo reaction product to STM7 49mer hairpin oligomers is varied. Product formation is maximal with an 8:1 ratio of STM7 acceptor to 5' thiophosphate donor DNA and by addition of extra ATP (0.5 mM) and ligase (60 units) midreaction | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AP endo | - |
Homo sapiens |
apurinic/apyrimidinic endonuclease | - |
Homo sapiens |
HAP1 | - |
Homo sapiens |
Ref1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
DNA containing apurinic/apyrimidinic sites | 5' cleavage of a reduced AP site | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |