Cloned (Comment) | Organism |
---|---|
expression in Sf9 cell | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000048 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C | Cricetulus griseus | |
0.000081 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme phosphorylated by casein kinase II, pH 7.5, 22°C | Cricetulus griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35500 | - |
x * 35500, SDS-PAGE | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | higher phosphorylation of APE1 in the expression host is correlated to a lower endonuclease activity. The phosphorylation of a higher activity batch of APE1 by casein kinase II decreases the endonuclease activity, and the dephosphorylation of APE1 by lambda phosphatase increases the endonuclease activity | Cricetulus griseus |
Purification (Comment) | Organism |
---|---|
recombinant protein | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
18-mer containing P33-labeled tetrahydrofuran | - |
Cricetulus griseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35500, SDS-PAGE | Cricetulus griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme phosphorylated by casein kinase II, pH 7.5, 22°C | Cricetulus griseus | |
5.7 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C | Cricetulus griseus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7200 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme phosphorylated by casein kinase II, pH 7.5, 22°C | Cricetulus griseus | |
12000 | - |
18-mer containing P33-labeled tetrahydrofuran | enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C | Cricetulus griseus |