Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Pyridinecarboxylic acid | - |
Paracoccus denitrificans | |
EDTA | 0.1 mM almost complete inhibition, activity restored by divalent cations | Paracoccus denitrificans | |
hydroxylamine | - |
Paracoccus denitrificans | |
Maleate | competitive | Paracoccus denitrificans | |
p-chloromercuribenzoate | GSH protects | Paracoccus denitrificans | |
Semicarbazide | - |
Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value varied with pH, optimum at pH 7.8 | Paracoccus denitrificans | |
0.43 | - |
erythro-L-beta-hydroxyaspartate | - |
Paracoccus denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
Mg2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
Mn2+ | restores activity after EDTA treatment | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Erythro-3-hydroxyaspartate | Paracoccus denitrificans | beta-hydroxyaspartate pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Paracoccus denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.15 | - |
- |
Paracoccus denitrificans |
Storage Stability | Organism |
---|---|
-15°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 60 days | Paracoccus denitrificans |
2°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 10 days | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Erythro-3-hydroxyaspartate | beta-hydroxyaspartate pathway | Paracoccus denitrificans | ? | - |
? | |
Erythro-DL-beta-hydroxyaspartate | irreversible, enzyme is highly substrate specific using only the L-isomer | Paracoccus denitrificans | Oxaloacetate + NH3 | - |
? | |
erythro-L-beta-hydroxyaspartate | - |
Paracoccus denitrificans | oxaloacetate + NH3 | - |
? | |
additional information | enzyme does not use threo-DL-beta-hydroxyaspartate, erythro and threo-DL-beta-hydroxy-beta methylaspartate, allo D-isomers of serine, threonine and allothreonine, D and L-aspartate, and isomers of tartrate | Paracoccus denitrificans | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Paracoccus denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Paracoccus denitrificans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | rapid loss of activity below pH 6 and above pH 10 | Paracoccus denitrificans |
7.5 | 9.5 | most stable | Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | activates | Paracoccus denitrificans |