Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Buckleyzyma aurantiaca | |
2-mercaptoethanol | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Rhodotorula glutinis | |
ascorbic acid | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Buckleyzyma aurantiaca | |
ascorbic acid | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Rhodotorula glutinis | |
EDTA | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Buckleyzyma aurantiaca | |
EDTA | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Rhodotorula glutinis |
General Stability | Organism |
---|---|
enzyme stabilization by 20% glycerol, 2-mercaptoethanol at 10 mM, 5 mM EDTA, and 2% ascorbic acid or 5 mM EDTA + 2% ascorbic acid | Buckleyzyma aurantiaca |
glycerol stabilizes the enzyme | Rhodotorula glutinis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-phenylalanine | competitive inhibition | Buckleyzyma aurantiaca | |
D-phenylalanine | competitive inhibition | Rhodotorula glutinis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Buckleyzyma aurantiaca | |
additional information | - |
additional information | Michaelis-Menten kinetics | Rhodotorula glutinis | |
0.25 | - |
L-phenylalanine | - |
Rhodotorula glutinis | |
1.33 | 1.75 | L-phenylalanine | in presence or absence of D-phenylalanine | Buckleyzyma aurantiaca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | Buckleyzyma aurantiaca | - |
(E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | Rhodotorula glutinis | - |
(E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | Buckleyzyma aurantiaca KM-1 | - |
(E)-cinnamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Buckleyzyma aurantiaca | - |
- |
- |
Buckleyzyma aurantiaca KM-1 | - |
- |
- |
Rhodotorula glutinis | Q2VMT1 | fragment | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.07 | 2.5 | - |
Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Buckleyzyma aurantiaca | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Buckleyzyma aurantiaca KM-1 | (E)-cinnamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-phenylalanine ammonia-lyase | - |
Buckleyzyma aurantiaca |
L-phenylalanine ammonia-lyase | - |
Rhodotorula glutinis |
PAL | - |
Buckleyzyma aurantiaca |
PAL | - |
Rhodotorula glutinis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Buckleyzyma aurantiaca |
30 | - |
assay at | Rhodotorula glutinis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
15 min, loss of 50% activity | Buckleyzyma aurantiaca |
57 | - |
15 min, loss of 50% activity | Rhodotorula glutinis |
65 | - |
10 min, loss of activity | Buckleyzyma aurantiaca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
assay at | Buckleyzyma aurantiaca |
8.9 | - |
assay at | Rhodotorula glutinis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.55 | - |
most stable at | Buckleyzyma aurantiaca |
6.55 | - |
most stable at | Rhodotorula glutinis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Buckleyzyma aurantiaca | |
additional information | - |
additional information | inhibition kinetics | Rhodotorula glutinis | |
3.3 | - |
D-phenylalanine | - |
Rhodotorula glutinis | |
3.38 | - |
D-phenylalanine | - |
Buckleyzyma aurantiaca |