Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H123A | only 1% of activity remaining compared to wild type, contributes to stabilization of pyridoxal 5'-phospahte | Escherichia coli |
H55A | no effect on activity | Escherichia coli |
R379A | less than 1% of activity remaining compared to wild type | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | Escherichia coli | - |
selenide + L-alanine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | - |
Escherichia coli | selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | enzyme bound pyridoxal 5'-phosphate serves as acceptor | Escherichia coli | selenide + L-alanine + acceptor | - |
? | |
additional information | traces of activity with L-cysteine | Escherichia coli | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Escherichia coli |